半夏倒苗前后的细胞组织结构

Structure of cell and histology around sprout tumble of Pinellia ternata

关键词:  半夏  倒苗  细胞组织学结构

Key words:  Pinellia ternata  sprout tumble  structure of cell and histology

摘要:

探讨高温胁迫下半夏倒苗前后的组织学结构的变化,为揭示半夏倒苗的生物学机理提供参考依据。在半夏植株高15 cm左右时,给予(32±1)℃的高温胁迫,于不同胁迫天数取半夏叶柄,经爱氏苏木精染液染色,用石蜡切片法制片,在显微镜下观察并照相。结果表明,随着高温胁迫时间的延长,半夏叶柄的维管组织结构被破坏越来越严重,细胞壁、细胞膜破裂,原生质外渗,核膜破裂,核仁溢出,有的细胞内核仁消失。

Abstract:

To study the change of Pinellia ternata around sprout tumble under high temperature stress and explore the biological mechanism about sprout tumble, high temperature stress was given when the petiole of Pinellia ternate was 15 cm high. At different days after treatment, the petiole was cut into 3-5 mm, then used for paraffin microtomy. The slices were observed under microscope and pictures were taken. The results were as follows: with the stress time increasing, the fibrovascular tissue structure of petiole was destroyed more seriously, the vascular bundles and cell membrane were broken, the protoplasm spilled over, and the nucleolus got out and even disappeared in some cells.