影响杜仲悬浮细胞生产桃叶珊瑚甙因素的研究

Studies on factors affecting aucubin production from the suspension cell of Eucommia ulmoides

关键词:  杜仲  桃叶珊瑚甙  外源激素组合  营养添加物

Key words:  Eucommia ulmoides  aucubin  the exohormone combination  nutrients

摘要:

研究了外源激素2,4-D、NAA、6-BA、KT的不同组合以及营养添加物水解酪蛋白、酵母提取物对杜仲悬浮细胞生产桃叶珊瑚甙的影响。结果表明,在2.0 mg/L 2,4-D的培养基中分别添加1.0 mg/L NAA、0.5 mg/L 6-BA、0.5 mg/L KT可提高桃叶珊瑚甙含量; 在培养基中添加水解酪蛋白和酵母提取物均能促进桃叶珊瑚甙的合成,以添加100 mg/L 水解酪蛋白和15 mg/L 酵母提取物时其含量最大。初步得出桃叶珊瑚甙合成的最适培养条件为添加2.0 mg/L 2,4-D+1.0 mg/L NAA+0.5 mg/L 6-BA+0.5 mg/L KT+100mg/L水解酪蛋白+15mg/L 酵母提取物的MS培养基。

Abstract:

Factors affecting aucubin production from the suspended cell of Eucommia ulmoides,such as the different exohormone combination of 2,4-D,NAA,6-BA,KT and nutrients including casein hydrolysate and yeast extract,were investigated. The results showed that the production of aucubin could be improved in media with 2.0 mg/L 2,4-D,1.0 mg/L NAA,0.5 mg/L 6-BA and 0.5 mg/L KT. Nutrients including casein hydrolysate and yeast extract could improve the production of aucubin,the optimal concentration was 100 mg/L casein hydrolysis and 15 mg/L yeast extract. Therefore,the optimal conditions for the production of aucubin were MS media with 2.0 mg/L 2,4-D,1.0 mg/L NAA,0.5 mg/L 6-BA,0.5 mg/L KT,100 mg/L casein hydrolysate and 15 mg/L yeast extract.