长梗黄精挥发油的化学成分及其生物活性

叶红翠<sup>1; 2</sup>; 张小平<sup>1*</sup>; 高贵宾<sup>1</sup>; 蒋继宏<sup>2</sup>

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长梗黄精挥发油的化学成分及其生物活性
叶红翠^1,2, 张小平^1*, 高贵宾¹, 蒋继宏²
1.安徽师范大学生命科学学院生物环境与生态安全安徽省高校重点实验室, 安徽芜湖 241000;2.徐州师范大学江苏省药用植物生物技术重点实验室, 江苏徐州 221116

摘要:

利用气相色谱-质谱联用仪对长梗黄精水溶性挥发物成分进行分析,共分离出30个组分,经检索鉴定出17个化合物,占总挥发油的85.297%,其主要成分为:1,2-邻苯二甲酸二异辛酯(41.047%); 并采用杯碟法和Alamar blue法检测该挥发油的抑菌和抗肿瘤生物活性,测得其对金黄色葡萄球菌、红酵母有较强的抑制能力; 对人非小细胞肺癌(NCI-H460)并无明显的抑制作用。

关键词: 长梗黄精挥发油 GC-MS 生物活性

DOI：

分类号:Q946.8

Fund project:

Chemical constituents and biological activity of volatile oil from the tubers of Polygonatum filipes

YE Hong-Cui^1,2, ZHANG Xiao-Ping^1*, GAO Gui-Bin¹, JIANG Ji-Hong²

1.Anhui Provincial Key Laboratory of Biotic Environment and Ecological Security, College of Life Sciences, Anhui Normal University, Wuhu 241000, China;2.Key Laboratory of Biotechnology for Medical Plant of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, China

Abstract:

The constituents of volatile oil from the tubers of Polygonatum filipeswere analyzed by GC-MS. About 30 components were separated and 17 compounds were identified,showing that 85.297% of the total contents and its main chemical constituent was 1,2-Benzenedicarboxylic acid,diisooctyl ester; biological activity of the volatile oil was screened with microbiological method and Alamar Blue method. The results showed that S.aureus and R.glutinis were sensitive to the volatile oil which couldn't obviously inhibit the growth of cultured tumor cells.

Key words: Polygonatum filipes volatile oil GC-MS biological activity