| 摘要: |
| 利用冰冻切片法结合间接免疫荧光标记技术对割手密茎尖细胞有丝分裂过程中微管骨架的变化进行了研究。结果表明:在割手密茎尖细胞有丝分裂过程中存在4种循序变化的典型微管列阵,即周质微管、早前期微管带、纺锤体微管及成膜体微管。在割手密初生增粗分生组织细胞中观察到的大多数是周质微管列阵,很少观察到其它3种典型的微管列阵,这可能这是割手密茎较小的原因之一。 |
| 关键词: 割手密 初生增粗分生组织 微管骨架 有丝分裂 |
| DOI： |
| 分类号:Q945.6 |
| Fund project: |
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| Change of microtubule cytoskeleton on mitosis in the shoot apex of Saccharum spontaneum |
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LI Zhi-Gang1*, LI Su-Li1, ZHANG Rong-Hua1,
YANG Li-Tao1, LI Yang-Rui2
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1.College of Agriculture, Guangxi University, Nanning 530005, China;2.Guangxi
Academy of Agricultural Sciences, Nanning 530007, China
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| Abstract: |
| The microtubule cytoskeleton was investigated by using cryosectioning and indirect immunofluorescence staining in the shoot apex of Saccharum spontaneum. The results indicated that:there were four basic types of microtubule arrays:cortical microtubule,preprophase-band,spindle and phragmoplast microtubule arrays. Cortical microtubule arrays were predominant in the primary thickening meristem of S.spontaneum,while other three arrays were rarely observed. It may be one of the reasons why S.spontaneum has a narrower stem than other cultivars. |
| Key words: Saccharum spontaneum primary thickening meristem microtubule cytoskeleton mitosis |
