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| 猕猴桃实生苗再生体系的建立 |
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周玲艳1,2, 秦华明3, 赖幸韵1, 陈楚欢1, 梁 红1*
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1.仲恺农业工程学院 生命科学学院, 广州 510225;2.华南农业大学生命
科学学院, 广州 510624;3.暨南大学理工学院, 广州 510623
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| 摘要: |
| 以成熟饱满的美味猕猴桃种子发芽获得实生苗,分别以实生苗的茎段、叶柄和叶片为材料建立了再生体系。结果表明:种子以2.5 mg/L的赤霉素(GA3)处理8 h较适合; 以培养基发芽较为适合; 茎段、叶柄和叶片的愈伤组织的诱导率均为100%,且茎段、叶柄比叶片容易脱分化,但叶片的平均出苗率最高。再生苗在移栽5d后,开始长出新叶,10 d后就能完全适应外界环境。 |
| 关键词: 猕猴桃 实生苗 外植体 再生体系 |
| DOI： |
| 分类号:Q943.1 |
| Fund project: |
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| Establishment of regeneration system of kiwifruit seedlings |
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ZHOU Ling-Yan1,2, QIN Hua-Ming3, LAI Xing-Yun1,
CHEN Chu-Huan1, LIANG Hong1*
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1.College of Life Science, Zhongkai Agriculture and Engiineering University, Guangzhou 510225, China;2.College of Life Science, South China Agricultural University, Guangzhou 510642, China;3.College of Science and Technology, Jinan University, Guangzhou, 510632, China
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| Abstract: |
| The full and mature seeds of kiwifruit were germinated to obtain the seedlings,and the regeneration system was established by using the stems,petioles and leaves as explants. The results showed that:the concentration of GA3 which was appropriate to treat the seeds was 2.5 mg/L and the treatment time was 8 h; the medium was more suitable for seed germination; the stems and petioles was easier to dedifferentiation than leaves,however,the average emergence rate of leaves was the highest. |
| Key words: kiwifruit seedling explants regeneration system |
