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| 铝胁迫对海莲幼苗保护酶系统及脯氨酸含量的影响 |
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马 丽 杨盛昌
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1.为探讨Al3+胁迫对海莲的影响,研究了10～50 mmol/L Al3+处理下海莲幼苗叶片和根系的过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)的活性以及可溶性蛋白质、丙二醛(MDA)和游离脯氨酸(Pro)含量的变化。结果表明,海莲幼苗能耐受50 mmol/L的Al3+胁迫处理,具有较高的耐铝性。但在50 mmol/L Al3+处理时,海莲幼苗叶片和根系的质膜系统膜脂过氧化加重,MDA含量增加;2.细胞活性氧代谢失衡。在保护酶系统中,Al3+处理促进了叶片中APX和POD活性的提高,降低了CAT的活性,SOD的活性呈下降趋势;3.海莲根部POD和SOD活性均显著提高,而CAT活性下降。25～50 mmol/L Al3+处理下,海莲叶片和根部可溶性蛋白质含量均显著下降;4.Pro的含量在叶片和根均有显著增加。
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| 摘要: |
| 海莲; 铝胁迫; 保护酶系统; 脯氨酸 |
| 关键词: 海莲 铝胁迫 保护酶系统 脯氨酸 |
| DOI： |
| 分类号:Q945 |
| Fund project: |
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| Effect of aluminium on protection enzyme system and proline of Brugiera sexangula seedlings |
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MA Li, YANG Sheng-Chang*
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Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystem,
School of Life Sciences, Xiamen University, Xiamen 361005, China
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| Abstract: |
| The activity of POD,CAT,SOD,APX,the content of soluble protein,MDA and proline in the leaves and roots of Brugiera sexangula were studied under 10-50 mmol/L AlCl3 treatments. The results showed that,B.sexangula had strong tolerance to high concentrations of Al3+,even 50 mmol/L of Al3+,but under this concentration,the membrane system in the leaves and roots were seriously damaged due to peroxidation,while the content of MDA increased and the active oxygen metabolism of the cells were unbalanced. In protection enzyme system,Al3+ treatments concentrated on the raising of APX and POD activity,reducing of CAT and SOD activity in leaves; for the roots,the POD and SOD activity were significantly increased,while the CAT activity were decreased. Under treatments of 25-50 mmol/L Al3+,in both leaves and roots,the soluble protein content decreased evidently but the Pro content increased. |
| Key words: Brugiera sexangula Al stress protection enzyme system proline |
