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| 黄连抗氧化活性研究 |
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袁王俊1,2, 李彩芳1,2, 丁秋瑾1, 康文艺1,2*
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1.河南大学 天然药物研究所, 河南 开封 475004;2.河南省天然药物与免疫工程重点实验室, 河南 开封 475004
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| 摘要: |
| 利用DPPH、FRAP和ABTS三种抗氧化活性分析方法对黄连植物不同部位的有机溶剂提取部分进行抗氧化评价,将所测定结果与Trolox进行比较,发现黄连植物不同部位抗氧化活性不同。其中,黄连须根的抗氧化活性最高; 同一部位中,乙酸乙酯和甲醇提取物抗氧化活性一般要高于石油醚提取物。在三种方法中,黄连不同部位的提取物清除自由基的能力均随浓度增大而增大; 三种方法之间有很好的相关性,以FRAP法与DPPH法相关性最好(r=0.9261,P<0.01)。 |
| 关键词: 黄连 抗氧化活性 DPPH FRAP ABTS |
| DOI： |
| 分类号:Q946 |
| Fund project: |
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| Studies on the antioxidant activity of Coptis chinensis |
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YUAN Wang-Jun1,2, LI Cai-Fang1,2, DING Qiu-Jin1, KANG Wen-Yi1,2*
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1.Institute of Natural Products, Henan University, Kaifeng 475004, China;2.Key Lab of
Natural Drug &3.Immune Engineering of Henan Province, Kaifeng 475004, China
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| Abstract: |
| Antioxidant activities of different extracts from different parts of Coptis chinensis were evaluated by the DPPH assay,FRAP assay and ABTS assay,and the results were compared to Trolox. The results showed that different parts had different activity,and fibrous root had the highest antioxidant activity; and different polar solvent extracts of the same part also demonstrated different antioxidant activity,ethyl acetate and methanol extracts generally had higher antioxidant activity than the petroleum ether extract. The antioxidant activity of the extracts from different parts of C.chinensis increased when the concentration of the extracts increased; there were good correlation among the three methods,and the values obtained by FRAP assay were correlated best with those obtained by DPPH assay(r=0.9261,P<0.01). |
| Key words: Coptis chinensis antioxidant activity DPPH FRAP ABTS |
