| 摘要: |
| 建立了高效液相色谱测定牙膏中柚皮苷含量的方法。采用的色谱条件:Hypersil BDS C18色谱柱(250 mm×4.6 mm,5 μm); 柱温为40 ℃; 以水(A相)和乙腈(B相),梯度洗脱程序为:0～15 min,10%～100% B; 流速为1.0 mL/min; 检测波长为283 nm; 进样量为20 μL。结果表明,柚皮苷的质量浓度在14.55～116.40 μg/mL范围内与峰面积呈良好的线性关系(r=0.9999),平均加标回收率为97.56%。该方法稳定、准确,重现性好,可作为牙膏中柚皮苷的含量测定和质量控制方法。 |
| 关键词: HPLC法 牙膏 柚皮苷 |
| DOI： |
| 分类号:Q946.83 |
| Fund project: |
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| Determination of naringin in toothpaste by high performance liquid chromatography |
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QIN Qing-Yun, LU Kai-Ling*
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Liuzhou Liangmianzhen Co.Ltd, Liuzhoui 545001, China
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| Abstract: |
| A high performance liquid chromatographic method was developed for determining the content of Naringin in toothpaste. The chromatographic analysis was carried out on a Hypersil BDS C18 column(250 mm×4.6 mm,5 μm). The mobile phase was water(A)and acetonitrile(B)with gradient elution(0～15 min,10%～100% B). The flow rate was maintained at 1.0 mL/min; The detection wavelength was set at 283 nm and the column temperature was controlled at 40 ℃,The sample injection volume was 20 μL. The results showed that the mass concentration of Naringin with good linearity vs. the peak area within ranges of 14.55～116.40 μg/mL,r=0.9999. The average recovery was 97.56%. The method was proved to be stable,reproducible and precise,It could be used for determination and quality control of naringin in toothpaste. |
| Key words: HPLC toothpaste Naringin |
