冰冻切片技术在海滨锦葵细胞学研究中的应用

Cryosectioning technique for cytological studies in Kosteletzkya virginica

关键词:  冰冻切片技术  海滨锦葵  蔗糖保护—液氮速冻法

Key words:  cryosectioning technique  Kosteletzkya virginica  sucrose protecting and liquid nitrogen quick frozen method

摘要:

为解决普通石蜡切片观察植物样品繁琐与耗时长的问题,利用改进的蔗糖保护—液氮速冻切片法,进行海滨锦葵不同器官细胞学研究。结果表明：(1)适合于海滨锦葵不同器官的最适蔗糖浓度不同,适于含水量较大的营养器官（根、茎和叶）的最适蔗糖浓度比含水量较小的花器官（柱头裂片、花柱、子房和花药）高。(2)利用最适蔗糖浓度的蔗糖保护-液氮速冻切片方法,获得了完整而清晰的海滨锦葵各器官组织细胞结构的切片;海滨锦葵具有典型的双子叶草本植物营养器官的组织细胞结构特征,花柱为闭合型花柱,子房为多室复子房。表明基于蔗糖保护-液氮速冻的冰冻切片技术在植物细胞学研究中具有广阔的应用前景。

Abstract:

To solve the fussy and long time consuming problems in observing general paraffin sections of plant specimens,the method combining sucrose protecting and liquid nitrogen frozen was used to study on cytology of different organs of Kosteletzkya virginica. The results showed:(1)It is different for the different organs of K.virginica to optimal sucrose concentration. Optimal sucrose concentrations for more water content organs(root,stem and leaf)are higher than that of less water content organs(stigma lobe,style,ovary and anther);(2)By the cryosectioning method of combining sucrose protecting of optimal concentration with liquid nitrogen quick frozen,the integral and clear cryosections were obtained,which could discriminate from organizational and cellular structures of different organs in K.virginica. This indicated K.virginica has typical structure characteristics of tissue cell of vegetative organs in dicotyledonous herbaceous plants. Its style belongs to solid style and ovary is multilocular ovaries. Cryosectioning technique combining sucrose protecting and liquid nitrogen quick frozen is useful for investigating plant cytology.