忽地笑愈伤组织培养条件对加兰他敏合成的影响

Effects of the culture conditions on galanthamine biosynthesis in Lycoris aurea calluses

关键词:  忽地笑  愈伤组织  加兰他敏  蔗糖  L 苯丙氨酸

Key words:  Lycoris aurea  callus  galanthamine  sucrose  L phenylalanine

摘要:

研究了忽地笑次生代谢产物加兰他敏的合成以及各种理化因子对加兰他敏合成的影响。结果表明，蔗糖是忽地笑愈伤组织培养的最佳碳源，而且在90 g/L浓度培养下加兰他敏含量最高，达到0068%；附加500 mg/L苯〖JP2〗丙氨酸前体物也可提高加兰他敏产量。酪氨酸和水杨酸不利于加兰他敏的积累。此外，2，4 D和浓度高于0.5 mg/L的NAA、IBA抑制加兰他敏的合成，但高浓度的6 BA却〖JP〗对加兰他敏的合成起促进作用。

Abstract:

The biosynthesis of galanthamine in callus culture of Lycoris aurea was studied. Some factors affected the biosynthesis of galanthamine were investigated. The results showed that sucrose was the best carbon source，and the concentration of 90 g/L was the most preferable for galanthamine production，which was reached to 0.068 %. Meanwhile，adding 500 mg/L L phenylalanine to medium，galanthamine content was also improved. Tyrosine and salicylic acid were not suitable for galanthamine accumulation in calluses. Besides，2，4 D inhibited the biosynthesis of galanthamine. Moreover，NAA and IBA above 0.5 mg/L could restrain the production of galanthamine，but 6 BA treated with high concentration promoted the biosynthesis of galanthamine.