水稻籼爪重组自交系PEG胁迫早期基因差异表达分析

Differentially expressed gene of indica/ javanica recombinant inbred line in rice at early stage of PEG stress

关键词:  水稻  籼爪重组自交系  PEG  干旱胁迫  差异表达基因

Key words:  rice  indica/javanica recombinant inbred line  PEG  drought stress  differentially expressed gene

摘要:

把从籼爪重组自交系中筛选出的最耐旱材料（47 274）和最敏感材料（47 299）在五叶一心时用20% PEG处理6 h，取叶片进行mRNA差异显示分析，获得10个差异表达的cDNA片段。利用反式Northern斑点杂交法去除6个假阳性片段，对4个阳性片段（DT DF1、DT DF2、DT DF3、DT DF4）测序后进行同源性分析，发现DT DF3的蛋白产物与核酸结合位点和富亮氨酸重复结构域类蛋白B高度同源，DT DF2与L 乳酸氧化酶部分序列有同源性，DT DF1没有找到同源蛋白质序列，DT DF4与一个假设蛋白高度同源。

Abstract:

The drought tolerant line and drought sensitive line screened from the population of indica/javanica recombinant inbred line were stressed with 20% PEG(Polyethyleneglycol) for 6 h at five leaf stage of rice. Ten of cDNA fragments were isolated by mRNA differentially display reverse transcription PCR(DDRT PCR)，but only four fragments were approved truth by reverse Northern dot blotting. These fragments named as DT DF1，DT DF2，DT DF3 and DT DF4 were sequenced and then analyzed by Blast program. The putative protein of DT DF3 was highly homologous with NBS LRR like protein B and that of DT DF2 was homologous with L lactate oxidase. The putative protein of DT DF1 was not found homologous protein，and that of DT DF4 was highly homologous with a hypothetical protein.