NAA与PP333对茉莉新梢部分生理生化作用的影响

Effects of NAA and PP333 on physiological and biological changes of Jasminum sambac

关键词:  NAA  PP333  茉莉  可溶性蛋白质  游离氨基酸  保护酶

Key words:  NAA  PP333  Jasminum sambac  soluble protein  free amino acid  protective enzymes

摘要:

在茉莉开花前期分别使用不同浓度NAA(20、50、100 mg/L)与PP333(100、300、500 mg/L)溶液均匀喷施于植株茎、叶片等生长部位，研究其对茉莉开花的影响，分析测定植株新梢生长部位的可溶性蛋白质、游离氨基酸含量以及保护酶活性等生理生化指标。结果表明:NAA处理使新梢徒长；PP333处理的茉莉，花蕾盛开期比对照提早4 d，浓度以300 mg/L为宜，其产量比对照增加12.95%，明显提高花蕾产量。茉莉经PP333处理后，新梢可溶性蛋白质和游离氨基酸含量在整个花芽生长过程中趋于相对稳定，而NAA处理后则使可溶性蛋白质和游离氨基酸含量变化幅度大。NAA与PP333处理后新梢中POD活性在花芽生长过程中稍有下降，NAA处理高于对照，而SOD活性则有上升趋势。经NAA和PP333处理后，其效应各不相同，而适宜浓度的PP333处理可以提高茉莉花蕾产量。

Abstract:

Different concentrations of NAA(20，50，100 mg/L) and PP333(100，300，500 mg/L) were sprayed on the stem and leaves of Jasminum sambac at early florescence stage，the effects on J.sambac flower buds formation were studied. The changes of soluble protein content，free amino acid content and protective enzymes activities in new shoots after treatment were observed. The results showed that new shoots excessively grew，florescence and growth phase were delayed after NAA treatment. While it was contrary that florescence was ahead of 4 d after PP333 treatment，the yield of flower increased remarkably to 12.95% than those of control, and the optimum concentration was 300 mg/L. The changes of soluble protein and free amino acid content were stable during flower bud growth stage after PP333 treatment. But it was on the contrary after NAA treatment. The POD activity decreased after NAA and PP333 treatments，and higher than those of control after NAA treatment. SOD activity increased after NAA and PP333 treatment. There were different effects after NAA and PP333 treatment，and suitable concentration of PP333 treatment could increase the yield of bud of J.sambac in this paper.