白花天目地黄的愈伤组织再生体系研究

Study on regeneration system from callus of Rehmannia chingii falbiflora

关键词:  白花天目地黄  幼嫩叶片  愈伤组织  植株再生

Key words:  Rehmannia chingii f.albiflora  young leaves  callus  plant regeneration

摘要:

白花天目地黄是天目地黄的一个优良变型，其资源稀少。本研究以白花天目地黄幼嫩叶片为外植体，探讨不同生长调节物质对其愈伤组织诱导及植株再生的影响。结果表明：MS+BA 1.5 mg•L^-1+IBA05 mg•L^-1是诱导叶片愈伤组织最佳的培养基；MS+BA 2.0 mg•L^-1+NAA 0.1 mg•L^-1培养基对不定芽分化的效果最好；不定芽增殖最适宜的培养基为MS+BA 2.0 mg•L^-1+IBA 0.2 mg•L^-1；其不定芽的最佳生根培养基为1/2MS+NAA 0.05 mg•L^-1；试管苗移栽成活率达到96.7%。同时，在此基础上探讨了白花天目地黄的园林绿化及对地黄属药用方面的利用前景。

Abstract:

Rehmannia chingii f.albiflora is a particular and scarce forma of R.chingii. In the present research，its young leaves were used as explants to study the effects of the growth regulators on callus induction and plant regeneration. The results indicated that the optimal media for induction and differentiation of callus were MS+BA1.5 mg•L^-1+IBA0.5 mg•L^-1 and MS+BA2.0 mg•L^-1+NAA0.1 mg•L^-1，respectively；the effective medium for cultivating adventitious buds was MS+BA2.0 mg•L^-1+IBA0.2 mg•L^-1; moreover，it’s found that the best inducing combination for root formation was 1/2MS+NAA0.05 mg•L^-1；the survival rate of test tube plantlets could reach 96.7 % after transplanting. In addition，the prospects of its gardening application and medicinal value were discussed.