摘要: |
引物选择、设计与应用策略是植物分子系统发育与进化研究的关键环节。本文综述了基因选择的原则、引物设计的技巧以及如何有效地利用所涉及的片段获取相应的PCR片段的方法。 |
关键词: 系统发育 进化 基因选择 引物设计 应用 |
DOI: |
分类号:Q941 |
Fund project: |
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Gene selection, primer design and their utility on the studies of plant molecular phylogeny and evolution |
WANG Yu-Guo
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Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of
Biodiversity Science, Fudan University(IBSFU), Shanghai 200433, China
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Abstract: |
Primer selection,design and utility are key and absolutely necessary steps on the studies of plant molecular phylogeny and evolution. Here the principles of gene selection,the skills of primer design and the efficient utility of the primers selected to obtain the needed PCR products are summarized. |
Key words: phylogeny evolution gene selection primer design utility |