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基于SRAP分析苎麻及近缘种的系统学关系
廖 亮1,2, 李同建1, 赵中伟1,2, 陈玉波1, 徐玲玲1*, 潘其辉3, 石庆华2*
1.江西九江学院 生命科学学院, 江西 九江 332000;2.江西农业大学 农学院, 南昌330045;3.江西省麻类科学研究所, 江西 宜春 336000
摘要:
苎麻与近缘种的系统学关系一直存在争议。利用26对多态性好的随机引物,对中国苎麻属的17种9变种的共27份材料进行SRAP分子标记分析。扩增结果是26对引物共扩出368条带,其中363条为多态性带,占98.64%。聚类分析表明:(1)腋球苎麻组的腋球苎麻和苎麻组的苎麻、贴毛苎麻聚在一支并靠近系统树的根部,支持王文采苎麻组起源于原始的腋球苎麻组观点。(2)苎麻和贴毛苎麻聚在一支,支持郭安平苎麻直接起源于贴毛苎麻的观点。(3)苎麻组的青叶苎麻、微绿苎麻和大叶苎麻组的多倍体类群聚在一支,可能是由于在种间杂交导致。
关键词:  中国苎麻属  SRAP  系统学关系
DOI:
分类号:Q949.737.5
Fund project:
Phylogenetic relationship of ramie and its wild relatives based on SRAP markers
LIAO Liang1,2, LI Tong-Jian1, ZHAO Zhong-Wei1, CHEN Yu-Bo1, XU Ling-Ling1*, PAN Qi-Hui3, SHI Qing-Hua2*
1.College of Life Science, Jiujiang University, Jiujiang 332000, China;2.College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China;3.Jiangxi Institute of Bast Fiber Crops, Yichun 336000, China
Abstract:
It was considered that there was disputation on the systematics of the genus Boehmeria,as well as the relationship among different sections in this genus. The phylogenetic relationship of 17 species and 9 varieties of Boehmeria was investigated using SRAP analysis based on a total of 27 samples. 368 bands were produced by SRAP technique using 26 primer pairs,of which 363(98.64%)bands were polymorphic.It was also indicated by clustering analysis that:(1)Boehmeria malabarica var.malabarica(sect.Boehmeria),B.nivea(sect.Tilocnide)and B.nivea var.nipononivea(sect.Tilocnide)were clustered into one branch and near the root of the phylogenetic tree,indicating that sect.Tilocnide originated from sect.Boehmeria;(2)B.nivea and B.nivea var.nipononivea were clustered into one branch,indicating that B.nivea originated directly from B.nivea var.nipononivea;(3)B.nivea var.viridula(sect.Tilocnide)and B.nivea var.tenacissima(sect.Tilocnide)were clustered together with the polyploid of sect.Duretia,which might have resulted from interspecific hybridization.
Key words:  Boehmeria  SRAP  phylogenetic relationship
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