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| 药用野生稻抗褐飞虱基因的RAPD标记研究 |
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梁肖仍1, 金 科1, 龙章德3, 申佩弘1, 蒋承建1, 武 波1,2*
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1.广西大学 生命科学与技术学院 微生物及植物遗传工程教育部重点实验室 广西亚热带生物资源
保护利用重点实验室, 南宁 530005;2.广西民族大学 化学与生态工程学院, 南宁
530007;3.广西中烟工业有限责任公司技术中心, 南宁 530005
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| 摘要: |
| 以药用野生稻1665(O.officinals)和栽培稻桂99远缘杂交的抗褐飞虱近等基因系B3F4分离群体为材料,利用随机引物对其抗褐飞虱基因进行了RAPD标记研究,获得3个与抗褐飞虱基因表现连锁的RAPD分子标记(S229703、S403783和S11591408)。采用MAPMAKER/EXP.Version 3.0分析,结果表明3个RAPD分子标记与抗褐飞虱基因属于同一连锁群,覆盖大小为18.1 cm,标记间平均距离为4.53 cm,为下一步水稻抗褐飞虱基因的精确定位和克隆奠定坚实的基础。 |
| 关键词: 药用野生稻 褐飞虱 抗褐飞虱基因 RAPD标记 |
| DOI： |
| 分类号:Q75 |
| Fund project: |
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| RAPD mapping of a brown planthopper(Nilaparvata lugens)resistance gene from Oryza officinalis |
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LIANG Xiao-Reng1, JIN Ke1, LONG Zhang-De3,
SHEN Pei-Hong1, JIANG Cheng-Jian1, WU Bo1,2*
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1.College of Life Science and Technology, Guangxi University, Key Laboratory of Ministry of Education for Microbial
and Plant Genetic Engineering, Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Nanning
530005, China;2.College of Chemistry and Ecology Engineering, Guangxi University for Nationalities, Nanning
530007, China;3.Technology Center, China Tobacco Guangxi Industrial Co.,Ltd., Nanning 530005, China
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| Abstract: |
| B3F4,a near-isogenic line(NIL)with Bph,was screened by distant hybridization of Oryza officinals 1665 and Oryza sativa Gui99,three molecular markers,S229703,S403783 and S11591408,linked to Bph,were obtained through RAPD analysis of B3F4 with random primers. The result of MAPMAKER/EXP. Version 3.0 analysis showed that these three molecular markers were belonged to the same linkage group with Bph,the covered range was 18.1cM and the average distance between markers was 4.53cM. This result will facillitate the futher accurate localization and cloning the Bph gene. |
| Key words: Oryza officinalis Nilaparvata lugens Bph RAPD marker |
