| 摘要: |
| 经过反复试验,摸索出一种提取高质量植物基因组DNA的方法:改良的4×CTAB法。以盐肤木叶片为实验材料,提取到高质量的基因组DNA,建立了酶切、连接、预扩增、选择性扩增的AFLP反应体系。通过两种引物组合“E+3/M+3”和“E+2/M+3”策略筛选出8对条带分辨率高、多态性好的引物组合,优化了盐肤木的AFLP银染反应体系,得到了清晰的AFLP指纹图谱,为盐肤木种群遗传多样性研究奠定了基础。 |
| 关键词: 盐肤木 DNA提取 4×CTAB法 AFLP技术 |
| DOI：10.3969/j.issn.1000-3142.2011.01.008 |
| 分类号:Q943.2 |
| Fund project: |
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| A modified method of genomic DNA extraction and establishment of AFLP systems in Rhus chinensis |
|
PANG Ya-Wen1, DUAN Li-Zhu1, SONG De-Ying2, REN Zhu-Mei1*
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1.School of Life Science, Shanxi University, Taiyuan 030006, China;2.Bureau
of Forestry of Wufeng County, Wufeng 443400, China
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| Abstract: |
| A modified method(4×CTAB)was used to extract the effective genomic DNA from the leaves of Rhus chinensis,and the systems of DNA restriction reaction,ligase reaction,pre-amplification and selective amplification reaction were established in this study. Using the two kinds of primer-combined methods(E+3/M+3 and E+2/M+3),8 pairs of primers were finally selected to amplify the genomic DNA of R.chinensis,and clear bands with high genetic polymorphism and difference were obtained. The present results provided a strong foundation for the further examination of population genetic diversity and structure of R.chinensis using AFLP method. |
| Key words: Rhus chinensis DNA extraction 4×CTAB method AFLP technology |
