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菜心下胚轴气孔的特征及其动态变化
徐 燕, 杨 颖, 玄晓丽, 王永飞
暨南大学 生物工程学系, 广州 510632
摘要:
以“油青60天”和“四九-19”2个菜心品种为材料,研究了菜心下胚轴气孔特征、气孔密度和气孔指数在2年内随不同发育时间的变化情况。结果显示:菜心种子萌发后第3天,下胚轴表皮上已有椭圆形或狭长形的气孔分布; 菜心下胚轴普通表皮细胞为长条形,排列紧密。“油青60天”和“四九-19”下胚轴的平均气孔密度均先下降后上升,分别在第27天和第21天达到最大值。随着幼苗的生长,“油青60天”下胚轴的平均气孔指数在萌发后第24天达最大值,与第3天的平均气孔指数差异显著; “四九-19”菜心下胚轴平均气孔指数在萌发后第21天达最大值,且与第12、15、27天的平均气孔指数差异性达到0.01水平。
关键词:  菜心  下胚轴  气孔  气孔密度  气孔指数
DOI:10.3969/j.issn.1000-3142.2011.01.025
分类号:Q944
Fund project:
Characteristics and dynamic changes of stomata on the hypocotyl of Brassica chinensis var.parachinensis
XU Yan, YANG Ying, XUAN Xiao-Li, WANG Yong-Fei*
Department of Biotechnology, Jinan University, Guangzhou 510632, China
Abstract:
The stomata distribution,stomata density,stomata index of Brassica chinensis var.parachinensis on hypocotyl surface were investigated with cultivars“youqing 60 days”and“sijiu-19”by light microscopy. The results showed that ordinary epidermal cells were usually long-flat in shape. Oval-shaped or strip-shaped stomata were observed on hypocotyl epidermis 3 days after seeds germination. The average stomata density on hypocotyl of“youqing 60 days”and“sijiu-19”decreased first and then increased during two years tests. The average stomatal density reached maximum on the 27th day and 21st day respectively. With the growth of seedlings,the average stomatal index on hypocotyl of“youqing 60 days”in the first 24 days increased to a maximum. Comparing with the first 3 days,the difference in stomatal index were significant. The average stomatal index on hypocotyl of “sijiu -19” reached maximum on the first 21 days after germination during the two years experiments,and comparing with that on the 12th day,15th day and 27th day,the difference between them were significant.
Key words:  Brassica chinensis var.parachinensis  hypocotyl  stomatal  stomata density  stomatal index
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