| 摘要: |
| 利用21个筛选出来的RAPD引物,对苏铁属21个种的22份材料进行分析,获得333个RAPD标记,利用NTSYS(V.2.10e)软件,建立了22份供试材料的UPGMA 聚类图,进而探讨了苏铁属21个种类间的亲缘关系。RAPD聚类分析结合形态学研究结果表明:多裂苏铁和叉孢苏铁的亲缘关系很近,聚为一类,多裂苏铁应为叉孢苏铁的一个亚种。西林苏铁、隆林苏铁、叉孢苏铁、尖尾苏铁、叉叶苏铁、长柄叉叶苏铁、多羽叉叶苏铁、长球果苏铁、贵州苏铁、四川苏铁、短叶苏铁、石山苏铁、宽叶苏铁、十万大山苏铁、元江苏铁、仙湖苏铁、海南苏铁、台湾苏铁、广东苏铁、滇南苏铁相互间的亲缘关系均较远,支持各自为独立的种。 |
| 关键词: 苏铁属 RAPD 聚类分析 亲缘关系 |
| DOI：10.3969/j.issn.1000-3142.2011.03.006 |
| 分类号:Q949.6 |
| Fund project: |
|
| Genetic relationships analysis in some species of Cycas in China by RAPD markersNONG Bao-Xuan1,2,4, HUANG Yu-Yuan3,4*, LIU Chi1 |
|
1.Rice research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 2.Key Lab of Guangxi
Crop Genetic Improvement and Biotechnology, Guangxi Academy of Agricultural Sciences, Nanning 530007, China;
3.Aricultural College, Guangxi University, Nanning 530004, China; 4.College of Life Sciences,
Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
|
|
In this paper,333 RAPD markers obtained from 22 materials of 21 species of Cycas were analyzed with the screened 21 RAPD primers. The genetic relationships constructed with UPGMA method of NTSYS software quite showed their linage relationship. According to the result of genetic relationship and the morphological traits analysis,it's concluded that C.multifida should be a sub-species of C.segmentifida,20 species that listed as follows:C.xilingensis,C.longlinensis,C.segmentifida,C.acuminatissima,C.micholitzii,C.longipetiolula,C.multifrondis,C.longiconifera,C.guizhouensis,C.szechuanensis,C.brevipinnata,C.miquelii,C.balansae,C.shiwandashanica,C.parvulus,C.fairylakea,C.hainanensis,C.taiwaniana,C.guangdongsnsis,C.diannanensis,should be a independent genetic units at species level.
|
| Abstract: |
| Cycas; RAPD; cluster analysis; genetic relationship |
| Key words: |
