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十四种金花茶组植物叶总黄酮含量比较
黄兴贤1,2, 邹 蓉2, 胡兴华2, 韦 霄2, 唐 辉2, 漆小雪2
1.广西师范大学 生命科学学院, 广西 桂林 541004;2. 广西壮族自治区 中国科学院 广西植物研究所, 广西 桂林 541006
摘要:
金花茶叶含有丰富的黄酮类化合物。以70%乙醇为提取溶剂,对14种金花茶叶中的黄酮进行超声波提取,并以芦丁为对照品,采用紫外分光光度法于273 nm处测定吸光度,得出各种金花茶叶总黄酮的含量并进行了比较。结果表明:不同种金花茶叶黄酮的含量差异较大,平果金花茶中新叶的总黄酮含量(22.011%)最高,而东兴金花茶中一年叶龄叶子总黄酮含量(1.518%)最低; 同种而不同叶龄的金花茶叶总黄酮含量变化较大。新叶的总黄酮含量较一年生叶和二年生叶的含量高。平果金花茶和柠檬黄金花茶叶中黄酮含量较高,具有潜在的研究与开发价值。
关键词:  金花茶组  超声波提取  紫外分光光度法  不同叶龄  总黄酮  含量测定
DOI:10.3969/j.issn.1000-3142.2011.03.028
分类号:Q946
Fund project:
Comparison of total flavonoids content in 14 species of Camellia sect.Chrysantha
HUANG Xing-Xian1,2, ZOU Rong2, HU Xing-Hua2, WEI Xiao2, TANG Hui2, QI Xiao-Xue2
1.College of Life Sience, Guangxi Normol University, Guilin 541004, China;2. Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China
Abstract:
There are rich flavonoids in leaves of Camellia sect.Chrysantha. The total flavonoids content in leaves of 14 species of Camellia sect.Chrysantha were extracted using 70% ethanol as extracting solvent by ultrasonic wave and were determined by ultraviolet spectrophotometry in this study,with rutin as a standard sample at 273nm. The content differences among species were analyzed. The results showed that the total flavonoids content in new leaves of C.pingguoensis(22.011%)was highest and the lowest was the one-year-old leaves of C.tunghinensis(1.518%). There were significant differences of the total flavonoids in different leaf ages of the same species. The total flavonoids content in new leaves is higher than that in one-year-old or two-year-old leaves. C.pingguoensis and C.limonia had the potential value of research and exploit with higher total flavonoids content.
Key words:  Camellia sect.Chrysantha  ultrasonic extraction  ultraviolet spectrophotometry  different leaf age  total flavonoids  content determination
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