| 摘要: |
| 利用RT-PCR技术从甜荞中克隆得到查耳酮合酶(CHS)的cDNA开放阅读框(ORF)序列,命名为FeChs,NCBI登录号为GU172166.1。该序列长1 179 bp,编码392个氨基酸,与其它植物CHS基因的同源性为78%～92%,其推导的氨基酸序列含有CHS高度保守的活性位点及CHS的标签序列GFGPG。 |
| 关键词: 甜荞 查耳酮合成酶基因 克隆 序列分析 |
| DOI：10.3969/j.issn.1000-3142.2011.05.019 |
| 分类号:Q943 |
| Fund project: |
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| Cloning and sequence analysis of chalcone synthase gene Chs from Fagopyrum esculentum |
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LI Cheng-Lei, ZHANG Xiao-Wei, WU Qi*, ZHAO Hai-Xia,
CHEN Hui, SHAO Ji-Rong
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College of Life Science, Sichuan Agricultural University, Ya'an 625014, China
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| Abstract: |
| The cDNA ORF sequence of chalcone synthase gene(FeChs)was amplified by RT-PCR from Fagopyrum esculentum. Its accession number is GU172166.1 on GenBank. The anlyasis indicated the cDNA sequence was 1 179 bp,encoding 392 amino acids and had a homlogy 78%-92% with other plants'CHS genes. The deduced amino acids sequence of FeChs contained the highly conserved catalytic active sites,and the typical Tag sequence of CHS with GFGPG. |
| Key words: Fagopyrum esculentum chalcone synthase gene cloning sequence analysis |
