应用RAPD和ISSR标记对24份花生栽培种材料进行遗传多样性分析

Genetic diversity in 24 peanut cultivars as revealed by RAPD/ISSR profiling

关键词:  花生  RAPD  ISSR  聚类分析  遗传多样性

Key words:  peanut  RAPD  ISSR  cluster analysis  genetic diversity

摘要:

利用RAPD引物和ISSR引物分析我国24份花生栽培种材料的遗传多样性。结果表明：所选的RAPD引物和ISSR引物中分别有13条引物和10条引物扩增出了清晰并可重复的条带，共扩增出123条带和87条带，平均每条引物扩增出9.5条带和8.7条带，其中多态性带分别占条带总数的47.15%和57.47%，平均每条引物扩增出4.7条和5.7条多态性带。在此基础上，根据Nei Li系数采用UPGMA法进行了聚类分析，可将24份花生材料分成4类:四粒红、汕油523、冀花2号、花育16和粤油7号等5个品种聚为一类；花育20、中花8号聚为一类；黑花生单独为一类；其余的花生聚为一类。RAPD和ISSR标记能够揭示花生栽培种的遗传多样性，在种质鉴定和遗传作图等方面具有一定应用潜力。

Abstract:

Genetic diversity in 24 accessions of cultivated peanut materials from China was evaluated based on RAPD/ISSR profiling. Of the RAPD primers and ISSR primers tested，13 and 10 primers produced a total of 123 and 87 repeatable，clear and readable bands，among which 47.15% and 57.47% were polymorphic，respectively. On average，a primer resulted in 9.5 and 8.7 bands，of which 4.7 and 5.7 were polymorphic. A dendrogram was constructed using UPGMA algorithm based on Nei and Li’s similarity coefficient，which divided the 24 peanut materials into 4 groups. Silihong，Sanyou 523，Jihua 2，Huayu 16 and Yueyou 7 were in one group. Huyu 20 and Zhonghua 8 clustered together. Heihuasheng formed a separated group，and the rest peanut materials tested fell in a group. RAPD and ISSR are useful for the genetic diversity studies of the cultivated peanut，and have potential in characterization of peanut germplasm and gene mapping.