扎龙芦苇遗传多样性ISSR分析

Genetic diversity of Phragmites australis populations from Zhalong based on ISSR analysis

关键词:  芦苇  居群遗传结构  遗传变异

Key words:  Phragmites australis  population genetic structure  genetic variation

摘要:

采用ISSR分子标记技术对扎龙湿地的5个野生芦苇居群进行了遗传多样性研究。从75条ISSR引物中筛选出10个特异性强、稳定性好的引物进行ISSR分析。共获得96个位点，其中多态位点94个，多态位点百分率为97.92%。PopGene分析结果表明：居群的平均多态位点百分率为56.46%，Shannon’s多样性指数(I)平均值为0.4783，具有较高的遗传多样性，居群间存在一定程度的基因流(Nm为0.9249)和遗传分化(Nei’s信息多样性指数平均值为0.3150，Gst平均值为0.3509)，居群内的基因多样度占总居群的64.91%，居群间占35.09%，表明芦苇居群的变异主要来源于居群内。聚类分析的结果表明：5个芦苇居群分为两类，克钦湖居群(K)和唐土岗居群(T)最近聚在一起，然后和稍远的局址居群(J)聚为一类；满代居群(M)和赵凯居群(Z)聚为一类。以上结果可为芦苇种质资源的保护和利用以及物种分化研究提供基础资料。

Abstract:

The genetic diversity of 5 wild Phragmites australis populations from Zhalong was estimated using inter simple sequence repeat（ISSR）markers，using ten specific and stable primers selected from 75 primers. A total of 96 sites were generated，94 of which(97.92%)were polymorphic. The data was analyzed by PopGene. The average percentage of polymorphicloci of populations was 56.46% and demonstrated high genetic diversity(the average value of Shannon’s index(I)was 0.4783). To some extent，both gene flow and genetic diversity were present among populations(Nm was 0.9249，average Nei’s index was 0.3150，and average Gst was 0.3509). The gene proportion of diversity reached 64.91% within population but was 35.09% among populations. This demonstrated that genetic variation of P.australis populations was mainly within populations. According to cluster analysis，the populations were classified into two large groups：the populations(K，T and J)grouped together，whereas populations(M and Z)grouped together. These results would supplement the information for protection and utilization of P.australis and also provide further data for the study of genetic variation and species differentiation of P.australis.