中低压制备色谱制备茜草蒽醌成分的研究

Study of anthraquinone component from rhizoma of Rubia cordifolia by preparative chromatogram of medium and low press

关键词:  中低压制备色谱  HPLC  IR  MS  1H NMR、13C NMR  茜草

Key words:  preparative chromatogram of medium and low press  HPLC  IR  MS  1H NMR,13C NMR  Rubia cordifolia

摘要:

建立新的中低压制备色谱技术，分离纯化茜草蒽醌单体。先采用高压液相色谱技术分析所分离产物的纯度，再用核兹共振谱、红外、质谱分析确证单体的化学结构。结果表明：用中低压色谱分离茜草的乙醇提取物而得到的4个单体，其纯度分别为99.87%、99.56%、99.42%、98.21%，结构鉴定为从石油醚萃取物中分离得到的单体为大叶茜草素，从氯仿萃取物中分离得到的单体为1，3，6 三羟基 2 甲基蒽醌，从乙酸乙酯萃取物中分离得到的单体分别为1，3，6 三羟基 2 甲基蒽醌 3 O (3′，6′ O 二乙酰基) α 鼠李糖（1→2） β D 葡萄糖苷和1，3，6 三羟基 2 甲基蒽醌 3 O 新橙皮糖苷。

Abstract:

A new technique of middle and low pressure preparative chromatogram was established，and the anthraquinone monomer from rhizoma of Rubia cordifolia were separated and purified. Purity of the compounds was analyzed by HPLC，and the structures of the compounds were confirmed by MS.1 1H NMR and 13C NMR.IR.The result showed that monocases were successfully obtained from ethanolic extract of Rubia cordifolia，with purities of 99.87%；99.56%；99.42 %；.98.21%. Mollugin was separated from petroleum ether extract；1，3，6 trihydroxy 2 methyl anthraquinone was separated from chloroform extract；1，3，6 trihydroxy 2 methyl anthraquinone 3 O (3′6′ O acety1) α rhamnose(1→2) β D glucoside and l，3，6 trihydroxy 2 methyl anthraquinone 3 O neohesperidoside were separated from acetic ether extract.