罗汉松遗传多样性的SCoT分析

韦泳丽1; $21; 2*; $21; $21

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罗汉松遗传多样性的SCoT分析
韦泳丽1，何新华1，2*，罗聪1，陈虎1
1．广西大学，南宁 530004;2．广西作物遗传改良与生物技术重点实验室，南宁 530007

摘要:

采用SCoT分子标记技术对8份罗汉松种质材料进行遗传多样性研究。结果表明，从80条引物中筛选出10条重复性好、条带清晰的引物进行PCR扩增，共产生136条带，其中多态性带122条(占88.97%)，8个罗汉松种质间的遗传相似系数范围在0.39～0.80说明罗汉松的遗传多样性丰富。利用UPGMA进行系统的聚类分析显示，将8份罗汉松材料分为2大类；主成分分析结果与聚类分析结果相一致。可见，利用SCoT分子标记可有效的分析罗汉松种质资源的遗传多样性，为罗汉松种质亲缘关系的鉴别和分类提供理论依据。

关键词: 罗汉松 SCoT 遗传多样性聚类分析

DOI：

分类号:Q949.66

Fund project:

Genetic diversity of podocarpus by SCoT markers

WEI YongLi1， HE XinHua1，2*， LUO Cong1， CHEN Hu1

1.Guangxi University， Nanning 530004， China;2.Guangxi Crop Genetic Improvement and Biotechnology Laboratory， Guangxi Academy of Agricultural Sciences， Nanning 530007， China

Abstract:

The genetic diversity of 8 podocarpus germplasms was analyzed by SCoT markers. The results showed that a total of 136 bands were produced by 10 primers，of which 122 bands(88.97%)were polymorphic，and the genetic similarity coefficients were between 0.39-0.80. So there were comparatively great genetic diversity among the podocarpus germplasms. A cluster analysis conducted with UPGMA also showed that the 8 podocarpus germplasms could be classified into 2 groups. The result of principal component analysis(PCA )was the same as the results of UPGMA cluster analysis. SCoT markers can be effectively used to evaluate the genetic diversity of podocarpus germplasms，thus providing a theoretical foundation for the identification and classification of podocarpus germplasms．

Key words: docarpus SCoT genetic diversity cluster analysis