罗汉果根多糖的分离纯化、结构鉴定及抗肿瘤活性研究

Studies on isolation、purification、structural identifi cation and its antitumor activity of polysaccharides from Momordica grosvenori swingle’s root

关键词:  罗汉果根  多糖  分离纯化  单糖组成分析  抗肿瘤

Key words:  Momordica grosvenori swingle’s root  polysaccharides  isolation and purification  structural identification  antitumor

摘要:

: 采用水提醇沉法提取、DEAE 52纤维素柱粗分离罗汉果根粗多糖（CPS），纸层析结合高效液相色谱分析CPS的单糖组成；同时研究CPS对小鼠H22皮下种植性肿瘤的影响。结果表明：采用DEAE 52纤维素柱粗分离得到5个多糖组分；CPS的单糖组成为：葡萄糖、阿拉伯糖、木糖，其中以葡萄糖为主；与模型对照组比较，CPS各剂量组无明显的抑制肿瘤生长作用（抑瘤率均低于50%）；与模型对照组比较，CPS各剂量组小鼠的胸腺指数显著提高，差异极显著（P＜0.01）；而脾脏指数显著降低，差异极显著（P＜0.01）。

Abstract:

Polysaccharide(CPS)of Momordica grosvenori swingle’s roots was extracted by water extracting alcohol precipitating method，and then was pufified by DEAE 52 cellulose. Their compositions were analyzed by paper chromatography and high performance liquid chromatography(HPLC). And the effect of CPS on H22 subcutaneous planting of mice was studied. Results:CPS was isolated into five fractionates by DEAE 52 cellulose column chromatography，its simple sugars composition included glucose，arabillose and xylose. Compared with model group，each dose group had no significant functions in suppressing tumor growth(inhibition rate＜50%)；Besides，Compared with model group，each dose group had improved influence on the index of thymus(P＜0.01)，but had reduced influence on the index of spleen(P＜001).