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广西红树植物桐花树种群遗传多样性分析
潘文1, 李元跃2*, 陈攀3, 周涵韬3, 林鹏3   
1.集美大学 生物工程学院, 厦门361021;2.集美大学 水产学院, 厦门 361021;3.厦门大学 生命科学学院, 厦门 361005
摘要:
采用RAPD PCR方法探讨广西3个不同生境下桐花树种群的遗传多样性和遗传分化。结果表明:3个不同生境桐花树RAPD扩增多态百分率为20.2%,3个不同生境桐花树种群两两之间的遗传距离分别为0.195、0.169、0.26,平均遗传距离为0.208。同一种群不同个体的扩增多态百分率最高为37.28%,其次为20.93%,最小的为19.32%。Shannon’s遗传多样性指数3个种群分别为0.331、0.225和0.17,其大小顺序与多态百分率的结果一致。种群内遗传多样性比率为62.3%,种群间遗传多样性比率为37.7%。说明广西3个不同生境的桐花树种群的遗传变异大部分存在种群内,种群间遗传变异较小。
关键词:  红树林  桐花树  种群  遗传距离  遗传多样性  RAPD
DOI:
分类号:Q948
Fund project:
Study on genetic diversity of mangrove plant Aegiceras corniculatum populations in Guangxi
PAN Wen1, LI YuanYue2*, CHEN Pan3, ZHOU HanTao3, LIN Peng3   
1.College of Bioengineering, Jimei University, Xiamen 361021, China;2.College of Fisheries, Jimei University, Xiamen 361021, China;3.School of Life Sciences, Xiamen University, Xiamen 361005, China
Abstract:
RAPD PCR markers were used to investigate the genetic diversity and genetic differentiation among 3 populations with different habitats of Aegiceras corniculatum in Guanxi. The results showed that percentage of polymorphic band was 20.2%,and genetic distances among 3 A.corniculatum populations were 0.195,0.169 and 0.26,respectively,and 0.208 on average. The percentage of polymorphic band and Shonnon’s diversity index among individuals within the same population had the same trend,and each was 37.28% and 0.331,20.93% and 0.225,19.32% and 0.17. A large proportion of genetic variance(62.3%)of A.corniculatum was among individuals within population,while only 37.7% genetic variance was among populations.
Key words:  angrove  Aegiceras corniculatum  populations  genetic distances  genetic diversity  RAPD
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