德保苏铁回归后几个生理指标的比较研究

Comparative study on several physiological characters among the re introduced population of Cycas debaoensis

关键词:  德保苏铁  回归引种  保护酶活性  脯氨酸  可溶性糖

Key words:  Cycas debaoensis  re introduction  defense enzyme activities  praline  soluble sugar

摘要:

以广西黄连山自然保护区内的德保苏铁幼苗为材料，研究了不同叶数苏铁幼苗叶片超氧化物歧化酶（SOD）、过氧化氢酶(CAT)、过氧化物酶(POD)活性以及丙二醛(MDA)、脯氨酸、可溶性糖和可溶性蛋白质含量的变化。结果表明:在7月至11月间，随着时间的推移，德保苏铁幼苗叶片的SOD、POD活性均呈递增趋势，CAT活性则先缓慢下降后又呈递增的趋势；MDA含量呈下降趋势；脯氨酸含量呈缓慢上升趋势；可溶性糖和可溶性蛋白质均呈持续积累趋势。综合分析结果表明：可将不同叶数苏铁幼苗分为两类：5叶植株单独为一类，环境适应能力强；3叶植株和4叶植株可聚为一类，环境适应能力相对较强。

Abstract:

Comparative study on the activity of superoxide dismutase(SOD)，catalase(CAT)，peroxidase(POD)，malonal dehyde(MDA)as well as the content of proline，soluble sugar and soluble protein in leaves of the seedlings of Cycas debaoensis in Huang lian shan Nature Reserve of Guangxi was carried out in the present paper. Results showed that the activity of SOD and CAT was increased in the whole development stage，while CAT activity decreased at earlier stage and then increased；MDA activity declined；proline showed a slow increasing tedency and the soluble sugar and soluble protein continuously accumulated. According to a comprehensive analysis，seedlings of C.debaoensis could be divided into two classes，plants with five leaves，which had a relatively higher environment adaptation；plants with three or four leaves merged into one class，which had a lower adaptability.