26种滇产植物提取物的体外抗耐药菌作用研究

Antimicrobial activities of 26 Yunnan plants extracts against clinical multi drug resistant pathogens in vitro

关键词:  滇产植物  提取物  耐药菌  抗菌活性  MIC/MBC

Key words:  Yunnan plant  extract  multi drug resistant pathogen  antimicrobial activity  MIC/MBC

摘要:

研究26种云南滇东南产植物80%乙醇提取物的体外抗耐药菌作用。制备植物的醇提物，用琼脂打孔法对其进行抗菌活性的筛选。通过微量稀释法测定提取物的最低抑菌浓度[minimum inhibitory concentration(MIC)]、最低杀细菌浓度[minimum bactericidal concentration(MBC)]及最低杀真菌浓度[minimum fungicidal concentration(MFC)]。结果表明：26种提取物中有9种对金黄色葡萄球菌（简称金葡菌）、大肠埃希菌、铜绿假单胞菌、白色念珠菌有不同程度的抑制活性；其中以红花荷、马蹄参、百花贝母兰、二色大包兰和光序肉实树提取物对耐甲氧西林金葡菌的抑菌作用最强，其MIC/MBC(mg/L)范围分别为512/2048～＞2048，512/2048～＞2048，256 512/2048～＞2048，512～1024/1024～＞2048和512～1024/＞2048。另外，肋果茶提取物对铜绿假单胞菌及白色念珠菌有较好的抑制作用，其对铜绿假单胞菌及其耐药菌株的MIC/MBC为512～2048/＞2048 mg/L；对白色念珠菌及其耐药菌株的MIC/MFC均为2048/＞2048 mg/L。26种植物提取物对大肠埃希菌的抑制作用均较弱。

Abstract:

To determine the in vitro antimicrobial activities of 80% ethanol extracts from 26 plants originated in southeast of Yunnan. The ethanol extracts of the collected plant samples were subjected to screen of the antibacterial and antifungal activity by the agar hole diffusion test. The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)or minimum fungicidal concentration(MFC)were determined by serial dilution with a standard broth microdilution method. The results showed that 9 of the 26 extracts were active with different potency against strains of Staphylococcus aureus，Escherichia coli，Pseudomonas aeruginosa and Candida albicans. The most active extracts against Staphylococcus aureus and methicillin resistant S.aureus(MRSA)were from Rhodoleia parvipetala Tong，Diplopanax stachyanthus Hand. Mazz，Sarcosperma kachinense Exell var.simondii Lam.et Royen，Coelogyne leucantha W.W.Smith and Sunipia bicolor. Their MICs/MBCs(mg/L)were 512/2048 ＞2048，512/2048 ＞2048，256 512/2048 ＞2048，512 1024/1024 ＞2048 and 512 1024/＞2048，respectively. Sladenia celastrifolia Kurz showed strong activity against P.aeruginosa and C.albicans，and the MICs/MBCs(MFCs)were 512 2048/＞2048 and 2048/＞2048 mg/L，respectively. All these plants showed weak inhibition against Escherichia coli.