桂林产白花丹茎不同月份白花丹醌含量测定

Determination of the plumbagin content in the stems of Plumbago zeylanica during different months in Guilin

关键词:  白花丹  白花丹醌  含量  高效液相

Key words:  Plumbago zeylanica  plumbagin  content  HPLC

摘要:

采用高效液相色谱法（HPLC），对桂林产的白花丹茎中不同月份的白花丹醌含量进行了测定。结果表明：在流动相为甲醇 水（70∶30），检测波长为254 nm的条件下，白花丹醌在0.00048～0.0240 mg·mL 1之间线性关系良好；回收率为96.9%～100.0%，白花丹茎中白花丹醌含量10月份最高，这时也是白花丹茎生物产量的高峰期，说明10月份是白花丹茎的最佳采收期。

Abstract:

The plumbagin content in stems of Plumbago zeylanica was determined during different months in Guilin，China，using RP HPLC，methanol and H2O (70∶30) as mobile phase and UV detection at 254 nm. Linear range over 0.00048-00240 mg·mL 1 of plumbagin was obtained. Recovery rate was within 969%-1000%. The content in stems of P.zeylanica was the highest in October when the plant was the most thriving and biggest in size. It was therefore considered that October was the best time for collecting stems.