南山茶果皮化学成分的研究

Research on the chemical constituents of the fruit peels of Camellia semiserrata

关键词:  山茶属  南山茶  化学成分

Key words:  Camellia  Camellia semiserrata  chemical constituents

摘要:

反复采用硅胶柱色谱法、Sephadex LH-20柱色谱法、ODS柱色谱法、反复重结晶等方法对南山茶果皮的化学成分进行分离纯化,并通过理化常数测定和波谱分析等方法进行结构鉴定。从南山茶果皮中分离并得到了10个化合物,分别鉴定为槲皮素(1)、山奈酚(2)、原儿茶酸(3)、山柰酚-3-O-β-D-葡萄糖基-(6→1)-2''',4'''-O-二乙酰基-α-L-鼠李糖基-(3→1)-2'''',3'''',4''''-O-三乙酰基-α-L-鼠李糖苷(4)、山柰酚-3-O-β-D-葡萄糖基-(6→1)-4'''-O-乙酰基-α-L-鼠李糖基-(3→1)-3''''-O-乙酰基-α-L-鼠李糖苷(5)、3-甲氧基鞣花酸(6)、鞣花酸(7)、羽扇豆烷醇(8)、β-谷甾醇(9)和胡萝卜苷(10)。化合物2、6、7均为首次从南山茶中分离得到,其中化合物6、7为首次从山茶属植物中分离得到。

Abstract:

The compounds were isolated from the peels of Camellia semiserrataby chromatography on silica gel column,Sephadex LH-20 column,ODS column and recrystallization,and the structures were determined on the basis of physicochemical evidence and ectroscopic analysis. Ten compounds were isolated and identified as quercetin(1),kaempferol(2),protocatechuicacid(3),kaempferol-3-O-〖(2'''',3'''',4''''-triacetyl)-α-L-rhamnopy-ranosyl(1→3)(2''',4'''-diacetyl)-α-L-rhamnopyranosyl(1→6)-β-D-glucopyranoside〗(4),kaempferol-3-O-〖(3''''-acetyl)-α-L-rhamnopyranosyl(1→3)(4'''-acetyl)-α-L-rhamnopyranosyl(1→6)-β-D-glucopyranoside〗(5),3'-O-methylellagic acid(6),ellagic acid(7),lupeol(8),β-sitosterol(9),daucosterol(10). Compounds 2,6 and 7 were all isolated from C.semiserrata for the first time. Compounds 6 and 7 were isolated from Camellia species for the first time.