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| 转拟南芥AtNPR1基因增强水稻对水稻白叶枯病和稻瘟病的抗性 |
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段承杰1,2, 罗荡平1,2, 罗雪梅1,2, 冯家勋1,2*
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1.亚热带农业生物资源保护与利用国家重点实验室, 南宁 530005;2.广西大学 生命科学与技术学院, 南宁 530005
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| 摘要: |
| AtNPR1基因是拟南芥系统获得抗性的一个重要调节基因,在拟南芥中过量表达AtNPR1基因能使拟南芥对细菌和真菌的抗性同时增强。为了研究在水稻中过量表达AtNPR1基因对水稻抗病性的影响,将该基因转入到广西主栽籼稻恢复系品种桂99中。经PCR验证得到了79株转基因植株,DNA斑点杂交表明ATNPR1基因已经整合到桂99染色体DNA中。Northern杂交和RT-PCR分析表明,AtNPR1基因在桂99中已经表达; 同时还检测了转基因植株对水稻白叶枯病和稻瘟病的抗性,结果表明转基因植株对该两种病害的抗性均显著增强。 |
| 关键词: AtNPR1基因 水稻 桂99 转化 抗病性 水稻白叶枯病菌 稻瘟病菌 |
| DOI：10.3969/j.issn.1000-3142.2012.06.016 |
| 分类号:Q789 |
| Fund project: |
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| Over-expression of Arabidopsis thaliana AtNPR1 gene in rice enhances the rice resistance to bacterial blight and blast diseases |
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DUAN Cheng-Jie1,2, LUO Dang-Ping1,2, LUO Xue-Mei1,2, FENG Jia-Xun1,2*
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1.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Nanning 530005,
China;2.College of Life Science and Technology, Guangxi University, Nanning 530005, China
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| Abstract: |
| The AtNPR1 gene is a key regulator of systemic acquired resistance(SAR)in Arabidopsis thaliana. Overexpression of AtNPR1 in A.thaliana enhanced resistance to both bacterial and fungal diseases. To investigate the effect of AtNPR1 over-expression in rice on its disease resistance, the gene was transformed as a restorer in Gui99 of Guangxi. PCR and DNA dot blot hybridization analyses revealed that 79 transgenic plants were obtained. Northern hybridization and RT-PCR analyses indicated that AtNPR1 gene was over-expressed in rice. The transgenic plants were challenged with Xanthomonas oryzae pv.oryzae(Xoo)and Magnaporthe grisea,the rice bacterial blight and fungal blast pathogens,respectively. They showed significantly enhanced resistance to Xoo and M.grisea. |
| Key words: AtNPR1 gene rice Gui99 transformation disease resistance X.oryzae pv.oryzae M.grisea |
