沙特番茄黄化曲叶病毒AC2基因编码蛋白的生物信息学分析

Bioinformatics analysis of coding protein of AC2 gene of Saudi tomato yellow leaf curl virus

关键词:  沙特番茄黄化曲叶病毒, AC2基因, 转录激活蛋白, 生物信息学

Key words:  Saudi tomato yellow leaf curl virus, AC2 gene, transcriptional activator protein, bioinformatics

摘要:

该试验旨在通过生物信息学方法分析沙特番茄黄化曲叶病毒AC2基因编码的转录激活蛋白TrAP(transcriptional activator protein)的结构及功能。该文通过在线软件预测和分析了番茄中AC2基因编码蛋白的一级结构、二级结构、磷酸位点、糖基位点、三级结构及其重要的功能; 通过最大似然法对不同物种 AC2基因编码蛋白序列构建系统发育树,进行系统发育分析。结果表明:番茄中AC2基因共编码135个氨基酸,且编码蛋白属于不稳定的亲水性蛋白质; 二级结构以无规则卷曲折叠为主,比例为58.52%; 该蛋白不存在跨膜结构域,属非跨膜蛋白且不存在信号肽; 该蛋白1～129个氨基酸区域为双生病毒超基因家族(Gemini_AL2 superfamily)结构域; 系统进化情况和植物分类学基本一致,沙特番茄双生病毒AC2基因与南非木薯间的亲缘关系最近。该基因编码蛋白结构与功能的分析为进一步研究AC2双生病毒基因家族奠定了基础,同时为番茄抗病在病虫害防治方面提供一种可行的思路。

Abstract:

This experiment aimed to analyze the structure and function of the transcriptional activator protein transcriptal activator protein(TrAP)encoded by the AC2 gene of the Saudi tomato yellow leaf curl virus by bioinformatics. In this study, primary structure, secondary structure, phosphate site, glycosylation site, tertiary structure and important functions of the protein encoded by AC2 gene in tomato were predicted and analyzed by online software. Phylogenetic trees were constructed by maximal likelihood method for the encoded protein sequences of AC2 genes from different species for phylogenetic analysis. The results were as follows: The AC2 gene encoded a total of 135 amino acids in tomato, and the encoded protein belonged to an unstable hydrophilic protein; The secondary structure was dominated by random coil folding, with a ratio of 58.52%; The protein did not have a transmembrane domain, it belonged to non-transmembrane protein and had no signal peptide; The 1-129 amino acid region of this protein was the Gemini_AL2 superfamily domain; The phylogenetics and plant taxonomy were basically the same, and the relationship between Saudi tomato yellow leaf curl virus AC2 gene and the South African cassava is recent. The analysis of the structure and function of the encoded protein provides a basis for further understanding of the AC2 geminivirus gene family, and provides a feasible approach for tomato disease resistance in pest control.