外源5 氨基乙酰丙酸对盐胁迫下决明幼苗生理特性的影响

Effects of ALA on physiological characteristics of Cassia obtusifolia seedlings under salt stress

关键词:  决明  外源5 氨基乙酰丙酸  盐胁迫  生理特性

Key words:  Cassia obtusifolia  ALA  salt stress  physiological characteristics

摘要:

为寻找提升决明幼苗耐盐性的方法，测量了不同处理下决明幼苗叶片的光合色素、超氧阴离子、丙二醛的含量，以及保护酶系统中SOD、POD、CAT的活性。结果表明，经150 mmol•L 1 NaCl处理，决明幼苗叶片的光合色素含量显著降低，而超氧阴离子、丙二醛的含量以及保护酶SOD、POD、CAT的活性显著升高。不同浓度的外源5 氨基乙酰丙酸在不同程度上增加了决明幼苗叶片的叶绿素、类胡萝卜素含量，进一步增强了保护〖JP2〗酶SOD、POD、CAT的活性，降低了超氧阴离子、丙二醛的含量。在外源5 氨基乙酰丙酸浓度为50 mg•L 1时，恢复效果最为明显(80.0 、196.4 和 114.1 U•g 1)，有效地缓解了盐胁迫对决明幼苗的伤害。

Abstract:

In order to get the method to improve the salt resistance of seedlings for Cassia obtusifolia under salt stress，physiological characteristics of C.obtusifolia seedlings were studied. Several physiological indexes of C.obtusifolia seedlings under salt stress like photosynthetic pigment，superoxide anion（O 〖ST4.〗2〖HT〗.），malondialdehyde(MDA)，superoxide(SOD)，peroxidase(POD) and catalase(CAT) were measured. The rate of decrease was relieved and the content of O 〖ST4.〗2〖HT〗.，MDA decreased. The activities of three enzymes including SOD，POD and CAT were increased by the treatment of ALA，and reached the maximin(80.0 U•g 1，196.4 U•g 1 and 114.1 U•g 1 respectively)with the concentration of 50 mg•L 1. ALA with concentration of 50 mg•L 1 could significantly alleviate the damages to the seedlings of C.obtusifolia under salt stress，and promote the salt resistance of the seedlings.