金花茶种子诱导人子宫颈癌HelaS3细胞凋亡及其作用机理的研究

Potential mechanism of Camellia chrysantha seeds induced apoptosis in human cervical cancer HelaS3 cells

Key words:  Camellia chrysantha  human cervical cancer  HelaS3 cells  apoptosis

摘要:

为探讨金花茶种子诱导人子宫颈癌HelaS3细胞凋亡的机制，以不同浓度金花茶种子乙醇提取物的正丁醇萃取物处理人子宫颈癌HelaS3细胞后，分别采用流式细胞仪、Western印记法等检测对细胞凋亡及Bax、Bcl 2、Bid等表达的影响。结果表明：金花茶种子乙醇提取物的正丁醇萃取物作用于人子宫颈癌HelaS3细胞后，细胞凋亡增加，Bax蛋白表达提高，Bcl 2蛋白表达降低，Bid蛋白活性减弱，线粒体膜电位下降及Caspase 8，Caspase 3活性增强。金花茶种子乙醇提取物的正丁醇萃取物为其重要的抗肿瘤活性有效部位，并可能通过激活Bax及Caspase 3，Caspase 8，抑制Bcl 2和Bid来诱导HelaS3细胞凋亡。

Abstract:

To investigate the effect of the seeds of Camellia chrysantha on apoptosis in human cervical cancer HelaS3 cells. Human HelaS3 cells were treated with the solution of BuOH fraction from the EtOH extract from the seeds of C.chrysantha at different concentrations. The apoptosis was detected by flow cytometry (FCM)，apoptosis associated proteins were determined by western blot. After treatment with the solution of BuOH fraction from the EtOH extract of the seeds of C.chrysantha on human HelaS3 cells，a significant enhancement of apoptosis was observed，the expression of Bax was enhanced，the expression of Bcl 2 and Bid decreased，and the decrease of mitochondrial membrane potential (MMP) and the activation of Caspase 8 and Caspase 3 were observed. The BuOH fraction from the EtOH extract of the seeds of C.chrysantha was the active anticancer fraction，which promoted human HelaS3 cells apoptosis possibly by activating Bax，MMP，Caspase 8 and Caspase 3，and inhibiting Bcl 2 and Bid.