| 摘要: |
| 培养基酸碱度是影响植物生长和次生代谢的重要因素之一。将生长稳定的国槐槐角愈伤组织在不同pH值的B5悬浮培养基中培养,比较其生长状况、生物量及苯丙氨酸转氨酶活性和黄酮类化合物的产量。结果表明:pH为6.6时,国槐细胞生长状况好,且不同pH下的苯丙氨酸转氨酶活性差异显著; 培养基呈弱酸性(即6.6±0.05)时,细胞黄酮类化合物含量较高。综合生长天数和产量,最佳的收获时期为继代后的第25～30天。 |
| 关键词: 槐角愈伤 pH值 PAL活性 黄酮类化合物 |
| DOI：10.3969/j.issn.1000-3142.2013.02.013 |
| 分类号:Q946.83 |
| Fund project: |
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| Effects of pH value on yield of flavonoids in callus tissues of Sophora japonica fruits |
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WEI Zhen-Yuan, TANG Ruo-Feng, WANG Jin-Fei,
DONG Qian-Qian, FAN Ya-Li, LIU Zhong-Hua*
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College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
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| Abstract: |
| The pH value of the medium is one of the important factors that affects plant growth and secondary metabolism. The stable growth callus tissues of Sophora japonica fruits were respectively cultured in B5 liquid medium with different pH values.The growth state and production,phenylalanine ammonialyase(PAL)activity and total yields of flavonoids were compared. The results showed 6.6 was suitable for the growth of callus tissues.PAL activity could be significantly affected by the pH value.Weak acid(6.6±0.05)medium was better for the most flavone compounds accumulation; in consideration of growth days and total yields,the best harvest period should be chosen at the 25th-30th day after subcultured. |
| Key words: callus tissues of fructus sophorae pH value PAL activity flavonoids |
