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| 桂西南早熟荔枝实生资源遗传多样性的ISSR分析 |
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沈庆庆1,3,4, 朱建华2*, 彭宏祥2, 何新华1
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1.广西大学 农学院, 南宁 530004;2.广西农业科学院 园艺研究所, 南宁 530007;3.玉林市农业科学研究所,
广西 玉林 537000;4.广西作物遗传改良生物技术重点开放实验室, 南宁 530007
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| 摘要: |
| 利用ISSR标记技术对83份早熟荔枝(品种)单株遗传多样性进行分析。筛选出多态性高的10条ISSR引物,共扩增出128条DNA条带,其中多态性带107条,多态性百分率为83.59%,表明桂西南早熟荔枝实生资源遗传多样性较丰富; 用NTSYS软件计算出这83份材料的DICE相似系数在0.64～0.95之间,遗传亲缘关系较近; 用UPGMA方法构建分子树状图,在相似系数0.75时,可将栽培品种与桂西南早熟实生单株区分开。各地区资源混杂聚类在一起,不能按地区单独聚类。 |
| 关键词: 早熟荔枝 实生资源 遗传多样性 ISSR |
| DOI：10.3969/j.issn.1000-3142.2013.02.017 |
| 分类号:S667.1 |
| Fund project: |
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| Genetic diversity of early-maturing seedling litchi resources in southwest Guangxi by ISSR |
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SHEN Qing-Qing1,3,4, ZHU Jian-Hua2*, PENG Hong-Xiang2, HE Xin-Hua1
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1.College of Agriculture, Guangxi University, Nanning 530004, China;2.Institute of Horticulture, Guangxi Academy
of Agricultural Sciences, Nanning 530007, China;3.Yulin Institute of Agricultural Sicence, Yulin 537000,
China;4.Guangxi Key lab of Crop Genetic Improvement and Biotechnology, Nanning 530007, China
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| Abstract: |
| The genetic diversities of 83 early-maturing litchi(variety)plants were examined by using ISSR markers in this study. Ten primers gave reproducible,polymorphic DNA amplification patterns with a total of 128 amplified bands. The percentage of polymorphic band was 83.59%,which showed that the early-maturing seedling litchi resources in southwest Guangxi had abundant genetic diversity. The genetic similarity ranged from 0.64 to 0.95 based on DICE. A dendrogram was constructed by UPGMA method,and the early-maturing litchi(variety)plants were distinguished from cultivar litchi at a similarity coefficient criterion of 0.75. No significant correlation between the dendrogram and geographical distance was revealed among the resources. |
| Key words: early-maturing litchi seedling litchi resources genetic diversity ISSR markers |
