| 摘要: |
| 木棉是分布于热带地区的重要经济树种。通过磁珠富集方法开发了木棉的SSR引物,并在采自于西南干热河谷的32个木棉个体中进行了多态性验证。结果表明:这些位点等位基因数量为2～6个,期望杂合度和观测杂合度范围分别为0～0.525和0.121～0.561。仅有一个位点显示Hardy-Weinberg平衡的偏离。这些微卫星标记的开发在木棉属中尚属首次,对于木棉的野生种群遗传多样性研究和木棉遗传资源利用上有很高的潜在价值。 |
| 关键词: 木棉 微卫星标记 遗传多样性 |
| DOI：10.3969/j.issn.1000-3142.2013.04.007 |
| 分类号:Q943 |
| Fund project: |
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| Developing SSR primers in Bombax ceiba(Bombacaceae) |
|
TIAN Bin, TIAN Xiang-Nan, XU Yu-Lan, MA Huan-Cheng*
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Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry
Administration, Southwest Forestry University, Kunming 650224, China
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| Abstract: |
| Bombax ceiba is a multipurpose tree species of tropical forests. Nine novel polymorphic microsatellite loci for B. ceiba were isolated and characterized through the combined biotin capture method. Markers were tested on 32 individuals collected from dry-hot valleys in southwest China. The number of alleles ranged from 2 to 6. The expected and observed heterozygosity values ranged from 0 to 0.525 and 0.121 to 0.561,respectively. One locus showed significant deviation from Hardy-Weinberg Equilibrium. No significant linkage disequilibrium was found. The microsatellite markers that were characterized in this study were the first to be developed for B. ceiba and would have great potential for different genetic studies of wild populations of this species. |
| Key words: Bombax ceiba microsatellite markers genetic diversity |
