MAP30蛋白结构功能的生物信息学研究

Research on bioinformatics of MAP30 protein structural function

关键词:  MAP30  抗HIV  生物信息学

Key words:  MAP30  anti-HIV  bioinformatics

摘要:

使用protparam、PHDhtm、PredictProtein等生物信息学在线服务器,对MAP30蛋白进行全面分析预测,研究MAP30蛋白具有的抗HIV活性,为临床应用提供抗科学依据和理论基础。结果表明:MAP30蛋白为稳定的碱性疏水蛋白,序列上存在三段跨膜螺旋结构和一段无序化位区域,肽链上的二硫键可使分子间形成聚集体,是一种分泌蛋白。MAP30蛋白序列包含信号肽、低复杂度区域和RIP样活性区域3个区域,具有细胞外被膜、异构酶、免疫应答三种功能。序列上分布着N-糖基化位点、N-豆蔻酰化位点、Shiga/ricin核糖体失活蛋白活性位点和多段蛋白激酶磷酸化位点。

Abstract:

It was designed to research the anti-HIV activities of protein MAP30 so as to provide scientific basis and theoretical basis for clinical application, to analyze and predict the protein MAP30 comprehensively using bioinformatics online servers, such as protparam, PHDhtm and PredictProtein. The results were as follows: MAP30 was stable alkali hydrophobin and the sequence thereof comprises three sections of transmembrane spiral structures and a section of disordering bit field; aggregates were formed among the molecules by the disulfide bond on peptide chain, therefore, it was a secretory protein. The protein MAP30 sequence contained signal peptide, a low complexity region and an RIP active region, therefore, it had three functions of cell envelope, isomerase and immune response. The sequence was distributed with N-glycosylation loci, N-myristoylation loci, Shiga/ricin ribosome inactivating protein loci and multi-zone protein kinase phosphorylation loci.