喀斯特典型地区烟管荚蒾AM真菌多样性研究

Diversity of arbuscular mycorrhizae of Viburnum utile in karst areas

关键词:  喀斯特  烟管荚蒾  AM真菌

Key words:  karst areas  Viburnum utile  arbuscular mycorrhizae

摘要:

烟管荚蒾是喀斯特地区灌木丛的主要组成植物。以茂兰喀斯特森林为采样地点,对烟管荚蒾AM真菌进行了分离、鉴定与多样性分析,并对优势菌种进行初步的接种研究。结果表明:烟管荚蒾根系的菌根侵染率为82.1%,从根际土壤分离到AM真菌2属16种,球囊霉属(Glomus)7种,无梗囊霉属(Acaulospora)9种。优势种为根内球囊霉(Glomus intraradices)、皱壁无梗囊霉(Acaulospora rugosa)和刺无梗囊霉(A. spinosa)。优势菌种接种紫花苜蓿(Medicago sativa),促生作用明显,并显著提高了SOD、POD和CAT酶活性。该研究对于探讨喀斯特地区AM真菌的多样性与独特性,筛选优良的宿主植物和与之高效共生的AM真菌具重要意义。

Abstract:

Viburnum utile is a major component of scrub in karst areas. To better elucidate arbuscular mycorrhizal fungal diversity,distribution associated with V. utile,and the effect of growth of Medicago sativa inoculated with the dominant species,we collected soil samples from Maolan karst forest. The results indicated that the infection rating of Viburnum utile by AMF was 82.1%. Sixteen species of arbuscular mycorrhizae belonging to two genera were isolated and identified from soil samples,including 7 species of Glomus and 9 species of Acaulospora. Meanwhile,Glomus intraradices,Acaulospora rugosa and A. spinosa were the dominant species. In addition,the growth and SOD,POD,and CAT activities of the Medicago sativa inoculated with dominant species were improved compared to the control without inoculation. These results would provide new insight into the AM fungal diversity and specificity,and play an important role in screening to identify excellent plant-AM fungus combination.