外源亚精胺对淹涝胁迫下黄瓜根HSP70表达的影响

Effects of exogenous spermidine on the expression of HSP70 in cucumber roots under waterlogging stress

关键词:  黄瓜  亚精胺  淹涝  HSP70

Key words:  cucumber  spermidine  waterlogging  HSP70

摘要:

采用逆转录聚合酶链式反应(RT-PCR)及蛋白免疫印迹杂交(Western Blot)技术,研究0.5 mmol/L亚精胺浸种的黄瓜幼苗在淹水胁迫下,根热激蛋白70基因(HSP70)mRNA和蛋白质的表达量的变化。结果表明:淹水胁迫使黄瓜根HSP70的mRNA和蛋白的表达呈现先上升后下降的趋势,在淹水4 h时,HSP70的mRNA和蛋白表达量均极显著高于未淹水处理; 亚精胺浸种的黄瓜根HSP70的mRNA和蛋白的表达量在24 h内呈一直上升的趋势,在淹水24 h时,HSP70的mRNA和蛋白表达量均极显著高于未淹水处理。淹涝胁迫下,亚精胺浸种的黄瓜根HSP70的mRNA和蛋白表达量在淹水12 h和24 h时极显著高于蒸馏水浸种。外源亚精胺能诱导淹涝胁迫下黄瓜幼苗根HSP70 mRNA和蛋白质的表达量的增加,缓解淹涝胁迫对黄瓜造成的伤害。

Abstract:

RT-PCR and Western Blot were adopted to study the effects of exogenous spermidine(0. 5 mmol/L)on HSP70 in mRNA and protein expression of cucumber roots under waterlogging stress. The results showed that under waterlogging stress,the expression of HSP70 in cucumber roots soaked with distilled water at mRNA and protein level increased and then decreased with the extension of waterlogging time,when the waterlogging time was up to 4 h,the expression quantity of HSP70 mRNA and protein were extremely higher compared with no waterlogging treatment; the expression of HSP70 in cucumber roots soaked with exogenous spermidine at mRNA and protein level increased within 24 h,when the waterlogging time was up to 24 h,the expression quantity of HSP70 mRNA and protein were extremely significant different compared with no waterlogging treatment. Under waterlogging stress,the expression of HSP70 mRNA and protein cucumber roots soaked with exogenous spermidine were extremely higher when the waterlogging time was up to 12 h and 24 h compared with soaked with distilled water. Exogenous spermidine could induce the expression of HSP70 and protein in cucumber roots increased under waterlogging stress,and could relieve the harm caused by waterlogging stress.