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| 油茶组培苗器官发生的形态学与解剖学研究 |
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吴幼媚1,2,3, 王鹏良1,2,3, 姚绍嫦4, 蒋 华5, 蒋 钢5, 陈晓明1,2,3*
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1. 广西林业科学研究院, 南宁 530002;2. 国家林业局 中南速生材繁育实验室, 南宁 530002;3. 广西优良用材林资源
培育重点实验室, 南宁 530002;4. 广西药用植物园, 南宁 530023;5. 全州县咸水林场, 广西 全州 541511
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| 摘要: |
| 以油茶岑软3号组培苗为研究材料,对其器官发生过程进行形态学和解剖学观察。结果表明:在选定的培养基上,外植体不经过愈伤组织,直接由器官诱导形成增殖芽,从而有效地保持了其遗传的稳定性。油茶增殖芽不定根由诱导的根原基发生,它起源于形成层薄壁细胞定向分化,不断向外进行伸长生长,并穿透愈伤组织而形成。这种不定根发生形式属皮部生根类型。油茶增殖芽单芽生根培养25 d后在不定根内形成次生维管形成层和木栓形成层,培养40 d以上根内的输导分子与茎内的输导分子相连。 |
| 关键词: 岑软3号油茶 器官发生 形态学 解剖学 |
| DOI：10.3969/j.issn.1000-3142.2013.06.005 |
| 分类号:Q944.53; S565.9 |
| Fund project: |
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| Morphology and anatomy of organogenesis from tissue culture plantlets of Camellia oleifera |
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WU You-Mei1,2,3, WANG Peng-Liang1,2,3, YAO Shao-Chang4,
JIANG Hua5, JIANG Gang5, CHEN Xiao-Ming1,2,3*
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1. Guangxi Academy of Forestry, Nanning 530002, China;2. Key Laboratory of Central South Fast-growing Timber
Cultivation, State Forestry Administration, Nanning 530002, China;3. Guangxi Key Laboratory of Superior Timber
Trees Resource Cultivation, Nanning 530002, China;4. Guangxi Botanical Garden of Medicinal Plant, Nanning
530023, China;5. Xianshui Forestry Farm of Quan County, Quanzhou 541511, Chian
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| Abstract: |
| The tissue culture plantlets of Camellia oleifera‘Cenruan No.3'were used as experimental materials to observe morphology and anatomy of organogenesis of microphyte. The results were as follows:the multiplied bud was directly from organogenesis way and otherwise from callus induced so that of the microphytes might be identical to Camellia oleifera‘Cenruan No. 3'in genetics. Any incubated root primordium was not found in multiplied buds. Induced root primordium which was originated form parenchma of cambium adventitious roots were emerged and grown. The type of adventitious root organogenesis belonged to cortex-rooting type. Vascular cambium and cork cambium were formed in induced adventitious root from multiplied singular bud after it was cultured in the rooting medium for 25 d and for more than 40 d conductive elements in root were connected to those of stems. |
| Key words: Camellia oleifera‘Cenruan No. 3' organogenesis morphology anatomy |
