用AFLP分析广东省鲜食橄榄品种资源遗传多样性

Genetic diversity analysis with AFLP on fresh Chinese olive cultivars resources in Guangdong Province

关键词:  AFLP  橄榄  遗传多样性  亲缘关系

Key words:  AFLP  fresh Chinese olive  genetic diversity

摘要:

从64对引物组合中筛选出6对谱带清晰、多态性高的引物组合,对63个橄榄品种扩增,共扩增出417条多态性电泳谱带,多态性比率达100%,揭示了橄榄品种的遗传多样性。利用UPGMA对供试橄榄品种聚类,建立AFLP聚类图。结果表明:63份材料之间的遗传相似性系数为0.0606～0.7619,相似系数为0.312时,可将供试材料分为7个品种群,其中第1群包括45个品种,以‘潮阳甜榄'为代表,还可细分成3个组; 第2群包括5个品种,以‘大纳甜'为代表; 第3群包括4个品种,以‘潮阳三棱'为代表; 第4群包括‘土甜'、‘文祠三棱'和‘早花三棱'3个品种; 第5群包括‘铁条'和‘细粒'两个品种; 第6群包括‘广太甜种'、‘西土'、‘鸡心'和‘车酸1号'4个品种; 第7群只有‘凤湖橄榄'1个品种,与其他品种群亲缘关系较远,可能发生变异。

Abstract:

Screened from 64 pairs of the AFLP primer,six pairs(E/AAC-M/CAT,E/AAC-M/CTT,E/ACA-M/CAA,E/AGG-M/CAA,E/ACC-M/CAA,E/ACT-M/CAT),which produced clear and reliable polymorphic bands,were selected for analysis genetic relationship and genetic diversity among the 63 Chinese olive cultivars. 417 amplification bands were produced with 100% polymorphic rate. It was indicated that there was rich genetic diversity in Chinese olive cultivar germplasm. The genetic relationship of the 63 Chinese olive cultivars was analyzed with software NTSYSpc-2.10a,and a UPGMA tree was established. According to this tree,the Chinese olive cultivars in this studied could be divided into 7 cultivar groups with a similar coefficient 0.312,the Group 1 delegated by ‘Chaoyang sweet olive' including 45 cultivars,could be divided into 3 types,the Group 2 delegated by ‘Danatian' including 5 cultivars,the Group 3 delegated by ‘Chaoyang sanleng' including 4 cultivars,the Group 4 including 3 cultivars,the Group 5 including 2 cultivars,the Group 6 including 4 cultivars,and the Group 7 including one cultivar only,that was ‘Fenghu olive',which had a long distance in relationship with other cultivars,maybe occurred variation in cultivation practice.