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| DOI：10.3969/j.issn.1000-3142.2014.01.007 |
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| Microsatellites primer development for Ottelia acuminata(Hydrocharitaceae), a submerged macrophyte endemic to southwestern China |
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LU Ran-Ran1,2, YANG Zhen-Yan1, TAO Cheng-Cheng1,2,
CHEN Shao-Tian1, JI Yun-Heng1*
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1. Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences,
Kunming 650201, China;2. University of Chinese Academy of Sciences, Beijing 100049, China
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| Abstract: |
| Ottelia acuminata(Gagnepain)Dandy(Hydrocharitaceae),is a submerged monocot endemic to southwestern China. Using the fast isolation by AFLP of sequences containing repeats(FIASCO)protocol,nine polymorphic microsatellite loci were identified by the genotyping of forty-five individuals from three natural populations. The number of alleles(NA)per locus within populations varied from one to three. The observed and expected heterozygosities ranged from 0.000 to 0.933 and 0.000 to 0.605,respectively. These microsatellite primers can be used in future studies on the phylogeography and ecological genetics of O. acuminata. |
| Key words: Hydrocharitaceae Ottelia acuminata polymorphic microsatellite |
