基于FTIR的三倍体罗汉果花粉直感效应研究

Xenia effect of triploid Siraitia grosvenorii based on fourier transform infrared spectroscopy

关键词:  傅里叶变换红外光谱  三倍体罗汉果  果实  花粉直感效应

Key words:  fourier transform infrared spectroscopy  triploid Siraitia grosvenorii  fruit  xenia effect

摘要:

以四个品系的三倍体罗汉果雌株为材料,用五种不同的二倍体雄花分别对其授粉,利用傅里叶变换红外光谱法(FTIR)测定了其子代果实的红外光谱,并运用主成分分析和聚类分析研究了授粉雄花对子代果实化学成分的影响。结果表明:不同雄花授粉后,雌株F302、F323和F322各子代果实红外光谱中1 050 cm^-1波数附近甜苷物质特征吸收峰的峰高有显著或极显著差异,雌株F311子代无籽果实的差异不显著; 同时五种授粉雄花分别对雌株F302、F323、F322和F311子代果实在主成分二维投影图和聚类图中的排序也有明显的影响,但对不同品系雌株子代果实的排序影响不同,从而说明三倍体罗汉果的甜苷物质含量和整体成分含量均有较为明显的花粉直感效应,并且存在品种特异性。

Abstract:

The triploid female Siraitia grosvenorii of four strains were pollinated with five different diploid male flowers of S. grosvenorii,respectively. The infrared spectrums of the fruits of their offspring were measured by fourier transform infrared spectroscopy(FTIR),and the effects of male flowers on chemical component of offspring fruits were studied using principal component analysis(PCA)and clustering analysis. The results showed that there was significantly diffirent between peak height of sweet glycosides material characteristic absorption peak in infrared spectroscopy 1 050 cm^-1(wave number)among females(F302, F323 and F322)offspring fruits,which was not significantly diffirent in female F311,while five kinds of female plants pollinated male F302, F323, F322 and F311 progeny fruit in two-dimensional projection of the main component and cluster diagram sort also had a significant impact,but for different strains of fruit sorting female plants affected offspring different,thus described triploid Mangosteen sweet glycosides ingredient content material content, and overall had a more significant effect Xenia, and there is variety-specific.