高效液相色谱法测定金钱菇多糖的单糖组成

伍善广; 冯学珍; 陈 颖; 蒙华琳; 陆 苑

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高效液相色谱法测定金钱菇多糖的单糖组成
伍善广, 冯学珍, 陈颖, 蒙华琳, 陆苑
1. 广西科技大学, 广西柳州 545006;2. 广东食品药品职业学院, 广东广州 510520

摘要:

采用高效液相色谱法,测定金钱菇多糖的单糖组成。用超声辅助提取金钱菇多糖,通过1-苯基-3-甲基-5-吡唑酮(PMP)衍生水解后的单糖,高效液相色谱法检测衍生物。结果表明:金钱菇多糖由甘露糖(Man)、核糖(Rib)、鼠李糖(Rha)、葡萄糖(Glc)、半乳糖(Gal)、木糖(Xyl)组成,其摩尔为1.00:0.90:0.91:28.03:1.58:0.11。该方法快速、简便、重现性好,可用于测定金钱菇多糖的单糖组成。

关键词: 金钱菇单糖柱前衍生化高效液相色谱法

DOI：10.3969/j.issn.1000-3142.2014.02.010

分类号:Q946

Fund project:

Analysis of monosaccharide compositions of Lentinus edodes polysaccharide by HPLC

WU Shan-Guang¹, FENG Xue-Zhen¹, CHEN Ying^2*, MENG Hua-Lin¹, LU Yuan¹

1. Guangxi University of Science and Technology, Liuzhou 545006, China;2. Guangdong Food and Drug Vocational College, Guangzhou 510520, China

Abstract:

Monosaccharide composition in Lentinus edode polysaccharides was determined by high performance liquid chromatography(HPLC). The L. edodes polysaccharide was extracted using ultrasonic-assisted extraction. Monosaccharide were derived by 1-phenyl-3-methyl-5-pyrazolone(PMP)and the PMP derivatives of monosaccharide were analyzed by HPLC. The results showed that L. edodes polysaccharide was composed of mannose,ribose,rhamnose,glucose,galactose and xylose and their molar ratio was about 1.00:0.90:0.91:28.03:1.58:0.11. The method was rapid,simple and reproducible and could be used to determine monosaccharide compositions of L. edodes.

Key words: Lentinus edodes monosaccharide pre-column derivatization HPLC