激素种类与浓度对鹿角杜鹃扦插繁殖的影响及其评价

Effects of hormone types and concentrations on cutting propagation of Rhododendron latoucheae and its evaluation

关键词:  鹿角杜鹃  扦插繁殖  激素种类与浓度  育苗性状  主成分分析

Key words:  Rhododendron latoucheae  cutting propagation  hormone types and concentrations  propagation traits  principle component analysis

摘要:

采用5种激素4个浓度的两因素完全随机区组设计,研究各因素及其组合对鹿角杜鹃扦插繁殖的7个插穗生根性状和5个扦插苗地上生长性状的影响,并运用主成分分析法对各处理组合的育苗效果进行了综合评价。结果表明:2个主因素对鹿角杜鹃扦插繁殖的大部分性状有显著影响,且表现为激素种类的影响大于浓度水平; 5种激素中,GA₃处理在愈伤率、腐烂率、生根率、老叶留存率与留存数、新梢率等性状上表现最佳,而IBA处理则在不定根数、最长不定根长、总根数、根系直径及新梢数、新梢长等性状上表现最优,两者为其扦插育苗的理想生根剂,其次为IAA处理,NAA和6-BA处理效果较差、不宜用于其扦插育苗; 4个浓度中,愈伤率、腐烂率及老叶留存率以低浓度(B₁)最佳,随着浓度升高效果下降; 其它9个性状则以中浓度(B₃)最优,高浓度(B₄)处理各育苗性状下降; 激素种类×浓度交互效应对总根数有极显著影响,对愈伤率、根系直径有显著影响,最佳浓度因激素种类而异。主成分综合评价表明,50 mg·L^-1 GA₃处理为最佳组合,其次为200、100 mg·L^-1IBA处理,可用于鹿角杜鹃产业化育苗。

Abstract:

Using cuttings of annual lignified branches of Rhododendron latoucheae as the material and using sand and soil(3:1,V/V)as the substrate,the effects of hormone types and concentrations and their combinations on 7 rooting traits and 5 above ground growth traits of R. latoucheae cutting propagation were studied,adopting a completely randomized design(CRD)with two factor of 5 hormone types and 4 concentration levels. And on this basis,the effects of different treatments combinations on cutting propagation were comprehensively evaluated by principal component analysis. The results showed that:Two main factors had significant influences on most cutting propagation traits,and hormone types had greater effect than concentration levels. Among 5 hormone types,GA₃ had the best effect on callusing rate,rotting rate,rooting rate,retention rate of old leaf,retention no of old leaf,and new shoot rate. And IBA had the best effect on number of adventitious root,length of longest adventitious root,number of total roots,diameter of root system,number of new shoot,and length of new shoot. Both of GA₃ and IBA were ideal hormones for R. latoucheae cutting propagation,followed by IAA,NAA and 6-BA had worse effect on propagation traits which should not be used for R. latoucheae cutting propagation. Among 4 concentration levels,low concentration(B₁)had the best effect for callusing rate,rotting rate and retention rate of old leaf,as the concentration became higher,it was less effective. Middle concentration(B₃)had the best effect for the other 9 propagation traits,and these traits decreased when treated with high concentration(B₄). The interaction effect of hormone types and concentrations had very significant influence on number of total roots,and it had significant influence on callusing rate and diameter of root system,and the best concentration level depended on hormone type. The results of comprehensive evaluation using principal component analysis showed that 50 mg·L^-1 GA₃ was the best treatment combination,followed by 200 mg·L^-1 IBA and 100 mg·