江西铅山红芽芋胚性愈伤组织的玻璃化法超低温保存及植株再生

Cryopreservation of Jiangxi Yanshan red bud taro (Colocasia esculenta var. cormosus cv. Hongyayu)embryogenic callus by vitrification and its plantlet regeneration

关键词:  江西铅山红芽芋  胚性愈伤组织  玻璃化法  超低温保存  植株再生

Key words:  Jiangxi Yanshan red bud taro(Colocasia esculenta var. cormosus cv. Hongyayu)  embryogenic callus  vitrification  cryopreservation  plantlet regeneration

摘要:

以江西铅山红芽芋胚性愈伤组织为材料,研究各种因素对其玻璃化法超低温保存的影响。结果表明:江西铅山红芽芋胚性愈伤组织玻璃化法超低温保存较佳的预培养条件为0.3 mol·L^-1蔗糖预培养3 d,较佳的60% PVS2装载时间为20 min,较佳的100% PVS2脱水条件为25 ℃脱水30 min,较佳的化冻温度为40 ℃,较佳的洗涤液蔗糖浓度为1.2 mol·L^-1,较佳的冻后培养条件为暗培养7 d再转到光周期中培养。红芽芋胚性愈伤组织包埋玻璃化超低温保存后的平均成活率约为70%。红芽芋胚性愈伤组织冻后再生苗没有发生形态学、生理学和细胞学的变异。

Abstract:

Using Jiangxi Yanshan red bud taro(Colocasia esculenta var. cormosus cv. Hongyayu)embryogenic calli as materials, the effects of various factors on its cryopreservation by vitrification were studied. The results showed that better preculture condition of Jiangxi Yanshan red bud taro embryogenic calli vitrification cryopreservation was 0.3 mol·L^-1 sucrose for 3 days. Better loading time was 60% PVS2 for 20 min. Better dehydration time of 100% PVS2 was 30 min at 25 ℃. Better thaw temperature was 40 ℃. Better sucrose concentration in washing media was 1.2 mol·L^-1. Better culture condition after cryopreservation was dark culture for 7 d and then transferred to the photoperiod. The average survival rate of embryogenic calli after cryopreservation by vitrification amounted to about 70%. No significant difference was observed in the morphological,physiological and cytological indexes of plantlets coming from control and cryopreserved embryogenic calli.