螺旋藻有效成分超临界萃取与分离研究

Study on the effective constituents of Spirulina platensis with supercritical fluid extraction and separation

关键词:  螺旋藻  超临界CO₂萃取  蛋白质  多糖

Key words:  Spirulina platensis  supercritical CO₂ extraction  protein  polysaccharide

摘要:

使用超临界CO₂萃取技术对钝顶螺旋藻粉进行萃取与分离,结果表明:超临界CO₂萃取的最优条件为压力20 Mpa,夹带剂使用量100 mL/100 g,温度50 ℃,萃取时间2 h; 利用凯氏定氮法及气相色谱法分别对超临界萃取处理过的螺旋藻中蛋白质和多糖进行研究与分析,结果表明:螺旋藻粉中蛋白质含量为49.39%,螺旋藻多糖中的单糖含量占多糖粗品的9.25%,主要组成为鼠李糖、木糖、甘露糖、葡萄糖、半乳糖等。

Abstract:

The effective constituents of the Spirulina platensis components were analysed and extracted used the supercritical CO₂ extraction. The optimal conditions of supercritical CO₂ were as follows: 20 MPa as extraction pressure,50 ℃ as extraction temperature,100 mL/100 g as entrainer,2 h as extraction time. And also analysis on Spirulina protein and polysaccharide by using Kjeldahl method and Gas Chromatography,the results showed that the protein content of Spirulina platensis was 49.39%,the content of Spirulina platensis monosaccharides in the polysaccharide was 9.25%. Most of monosaccharides was composed from rhamnose,xylose,mannose,glucose and galactose.