| 摘要: |
| 采用有机酸法水解制备蔗渣低聚木糖,通过单因素实验、正交试验研究了甲酸-乙酸比例、温度、水解时间、固液比等因素的影响,以水解率、总糖收率和聚糖收率为考察指标,得到有机酸法水解蔗渣制备低聚木糖的最优预处理条件为甲酸:乙酸=9:1、水解温度100 ℃、水解时间60 min、固液比1:7,在此条件下蔗渣水解率为47.78%,总糖收率20.57%,聚糖收率11.88%。HPLC检测结果显示:水解物中木二糖含量为17.69%,木三糖为11.23%,更高聚合度聚糖所占比例为29.42%,木糖为36.78%。半纤维素有机酸水解物可进一步通过木聚糖酶水解、分离制备低聚木糖。研究结果可为蔗渣制备低聚木糖新工艺提供科学依据 |
| 关键词: 蔗渣 有机酸 低聚木糖 |
| DOI：10.3969/j.issn.1000-3142.2014.04.015 |
| 分类号:Q946 |
| Fund project: |
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| Study on pretreatment conditions of organic acid to get xylooligosaccharide with bagasse |
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CHEN Hai-Shan1, LI Tao-Tao2, ZHOU Yü-Heng1,
CAI Ai-Hua1, QIN Xiang-Xiang1
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1. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guilin
541006, China;2. National Baise Senior High School, The Guangxi Autonomous Region, Baise 533000, China
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| Abstract: |
| Xylooligosaccharide of bagasse were hydrolyzed with organic acid by single factor and orthogonal experiments,through the research of the effecting factors such as the ratio,temperature,hydrolysis time and solid-to-liquid ratio of formic acid and acetic acid. With the hydrolysis rate,total sugar and polysaccharide yield as index,the optional pretreatment technological conditions with the hydrolysis of organic acid to get xylooligosaccharide were obtained as follows: formic acid:acetic acid=9:1,hydrolyzing temperature 100 ℃,hydrolyzing time 60 min and solid-to-liquid ratio 1:7,and under this condition,bagasse hydrolysis rate was 47.78%,total sugar yield 20.57%,and polysaccharide yield 11.88%. The results of HPLC test showed that xylobiose content was 17.69%,Xylotriose 11.23%,polysaccharide with higher polymerization degree 29.42% and xylose 36.78% in the hydrolyzate,and the hydrolyzate of Hemicellulose organic acid could be furtherly hydrolyzed with xylanase and be separated to obtain xylooligosaccharide. The result of this study would provide the scientific basis for new technology to get xylooligosaccharide with bagasse. |
| Key words: bagasse organic acid xylooligosaccharide |
